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http://purl.uniprot.org/citations/23803888http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/23803888http://www.w3.org/2000/01/rdf-schema#comment"

Background

β,β-Carotene 15,15'-monooxygenase (BCMO1) converts β-carotene to retinaldehyde. Increased β-carotene consumption is linked to antitumor effects. Retinoic acid reduces the invasiveness in cancer, through inhibition of matrix metalloproteinases (MMPs). In our studies of a mouse model that develops intestinal tumors after low dietary folate, we found reduced BCMO1 expression in normal preneoplastic intestine of folate-deficient tumor-prone mice.

Objective

Our goal was to determine whether BCMO1 expression could influence transformation potential in human colorectal carcinoma cells, by examining the effect of BCMO1 modulation on cellular migration and invasion, and on expression of MMPs.

Design

LoVo colon carcinoma cells were transfected with BCMO1 small interfering RNA (siRNA) or scrambled siRNA. Migration and invasion were measured, and the expression of BCMO1, MMP7, and MMP28 was assessed by quantitative reverse-transcriptase polymerase chain reaction. These variables were also measured after treatment of cells with retinoic acid, 5-aza-2'-deoxycytidine, folate-depleted/high-methionine medium, and β-carotene.

Results

Retinoic acid decreased the migration, invasion, and expression of MMP28 mRNA. Transfection of cells with BCMO1 siRNA inhibited BCMO1 expression, enhanced migration and invasion, and increased expression of MMP7 and MMP28. 5-Aza-2'-deoxycytidine decreased, whereas folate-depleted/high-methionine medium increased invasiveness. β-Carotene increased BCMO1 expression and reduced invasiveness with a decrease in expression of MMP7 and MMP28.

Conclusions

Inhibition of BCMO1 expression is associated with increased invasiveness of colon cancer cells and increased expression of MMP7 and MMP28. β-Carotene can upregulate BCMO1 and reverse these effects. These novel associations suggest a critical role for BCMO1 in cancer and provide a mechanism for the proposed antitumor effects of β-carotene."xsd:string
http://purl.uniprot.org/citations/23803888http://purl.org/dc/terms/identifier"doi:10.3945/ajcn.113.060996"xsd:string
http://purl.uniprot.org/citations/23803888http://purl.uniprot.org/core/author"Leclerc D."xsd:string
http://purl.uniprot.org/citations/23803888http://purl.uniprot.org/core/author"Rozen R."xsd:string
http://purl.uniprot.org/citations/23803888http://purl.uniprot.org/core/author"Deng L."xsd:string
http://purl.uniprot.org/citations/23803888http://purl.uniprot.org/core/author"Pham D.N."xsd:string
http://purl.uniprot.org/citations/23803888http://purl.uniprot.org/core/author"Levesque N."xsd:string
http://purl.uniprot.org/citations/23803888http://purl.uniprot.org/core/date"2013"xsd:gYear
http://purl.uniprot.org/citations/23803888http://purl.uniprot.org/core/name"Am J Clin Nutr"xsd:string
http://purl.uniprot.org/citations/23803888http://purl.uniprot.org/core/pages"413-422"xsd:string
http://purl.uniprot.org/citations/23803888http://purl.uniprot.org/core/title"beta,beta-carotene 15,15'-monooxygenase and its substrate beta-carotene modulate migration and invasion in colorectal carcinoma cells."xsd:string
http://purl.uniprot.org/citations/23803888http://purl.uniprot.org/core/volume"98"xsd:string
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