| http://purl.uniprot.org/citations/23810831 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23810831 | http://www.w3.org/2000/01/rdf-schema#comment | "The 5-HT₃ receptor is a cation selective member of the pentameric Cys-loop ligand-gated ion channels. While five subunits are known to exist, only two receptor subtypes have been significantly characterized: the homomeric receptor consisting of five A subunits and the heteromeric receptor containing both A and B subunits. The agonist recognition and activation of these receptors is orchestrated by six recognition loops three, A-C, on the principal subunit, and three, D-F, on the complementary subunit. In this study we have focused on the B loop of the principal subunit and loop D of the complementary subunit where aligned amino acids differ between the two subunits. A mutational analysis has been carried out using both 5-HT and m-chlorophenylbiguanide (mCPBG) to characterize receptor activation in the mutant receptors using two-electrode voltage clamp in Xenopus oocytes. The results show that the B loop W178I mutation of the 5-HT3A subunit markedly reduces the efficacy of mCPBG in both the homomeric and heteromeric receptors, while activation by 5-HT remains intact. Replacement of the D loop amino acid triplet RQY of the 5-HT3A subunit, with the aligned residues from the 5-HT3B subunit, QEV, converts 5-HT to a weak partial agonist in both the homomer and heteromer, but does not compromise activation by mCPBG. Exchange of the RQY triplet for the 5-HT3B subunit homologue, QEV, increases the Hill coefficient and decreases the EC₅₀ of this mutant when expressed with the wild type 5-HT3A subunit."xsd:string |
| http://purl.uniprot.org/citations/23810831 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.neuropharm.2013.06.017"xsd:string |
| http://purl.uniprot.org/citations/23810831 | http://purl.uniprot.org/core/author | "Dunn S.M."xsd:string |
| http://purl.uniprot.org/citations/23810831 | http://purl.uniprot.org/core/author | "Martin I.L."xsd:string |
| http://purl.uniprot.org/citations/23810831 | http://purl.uniprot.org/core/author | "Paulsen I.M."xsd:string |
| http://purl.uniprot.org/citations/23810831 | http://purl.uniprot.org/core/author | "Michaelson S.D."xsd:string |
| http://purl.uniprot.org/citations/23810831 | http://purl.uniprot.org/core/author | "Kozuska J.L."xsd:string |
| http://purl.uniprot.org/citations/23810831 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23810831 | http://purl.uniprot.org/core/name | "Neuropharmacology"xsd:string |
| http://purl.uniprot.org/citations/23810831 | http://purl.uniprot.org/core/pages | "398-403"xsd:string |
| http://purl.uniprot.org/citations/23810831 | http://purl.uniprot.org/core/title | "Importance of recognition loops B and D in the activation of human 5-HT(3) receptors by 5-HT and meta-chlorophenylbiguanide."xsd:string |
| http://purl.uniprot.org/citations/23810831 | http://purl.uniprot.org/core/volume | "73"xsd:string |
| http://purl.uniprot.org/citations/23810831 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23810831 |
| http://purl.uniprot.org/citations/23810831 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/23810831 |
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| http://purl.uniprot.org/uniprot/#_B3VRL5-mappedCitation-23810831 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23810831 |
| http://purl.uniprot.org/uniprot/#_B3VRM0-mappedCitation-23810831 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23810831 |
| http://purl.uniprot.org/uniprot/#_B3VRM5-mappedCitation-23810831 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23810831 |
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| http://purl.uniprot.org/uniprot/#_B3VRP0-mappedCitation-23810831 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23810831 |
| http://purl.uniprot.org/uniprot/#_B3VRP5-mappedCitation-23810831 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23810831 |
| http://purl.uniprot.org/uniprot/#_O95264-mappedCitation-23810831 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23810831 |
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