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| http://purl.uniprot.org/citations/23824292 | http://www.w3.org/2000/01/rdf-schema#comment | "The antibiotic blasticidin S (BlaS) is a potent inhibitor of protein synthesis in bacteria and eukaryotes. We have determined a 3.4-Å crystal structure of BlaS bound to a 70S⋅tRNA ribosome complex and performed biochemical and single-molecule FRET experiments to determine the mechanism of action of the antibiotic. We find that BlaS enhances tRNA binding to the P site of the large ribosomal subunit and slows down spontaneous intersubunit rotation in pretranslocation ribosomes. However, the antibiotic has negligible effect on elongation factor G catalyzed translocation of tRNA and mRNA. The crystal structure of the antibiotic-ribosome complex reveals that BlaS impedes protein synthesis through a unique mechanism by bending the 3' terminus of the P-site tRNA toward the A site of the large ribosomal subunit. Biochemical experiments demonstrate that stabilization of the deformed conformation of the P-site tRNA by BlaS strongly inhibits peptidyl-tRNA hydrolysis by release factors and, to a lesser extent, peptide bond formation."xsd:string |
| http://purl.uniprot.org/citations/23824292 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.1304922110"xsd:string |
| http://purl.uniprot.org/citations/23824292 | http://purl.uniprot.org/core/author | "Ermolenko D.N."xsd:string |
| http://purl.uniprot.org/citations/23824292 | http://purl.uniprot.org/core/author | "Ling C."xsd:string |
| http://purl.uniprot.org/citations/23824292 | http://purl.uniprot.org/core/author | "Korostelev A.A."xsd:string |
| http://purl.uniprot.org/citations/23824292 | http://purl.uniprot.org/core/author | "Svidritskiy E."xsd:string |
| http://purl.uniprot.org/citations/23824292 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23824292 | http://purl.uniprot.org/core/name | "Proc Natl Acad Sci U S A"xsd:string |
| http://purl.uniprot.org/citations/23824292 | http://purl.uniprot.org/core/pages | "12283-12288"xsd:string |
| http://purl.uniprot.org/citations/23824292 | http://purl.uniprot.org/core/title | "Blasticidin S inhibits translation by trapping deformed tRNA on the ribosome."xsd:string |
| http://purl.uniprot.org/citations/23824292 | http://purl.uniprot.org/core/volume | "110"xsd:string |
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