| http://purl.uniprot.org/citations/23831686 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23831686 | http://www.w3.org/2000/01/rdf-schema#comment | "Microtubules are highly dynamic αβ-tubulin polymers. In vitro and in living cells, microtubules are most often cold- and nocodazole-sensitive. When present, the MAP6/STOP family of proteins protects microtubules from cold- and nocodazole-induced depolymerization but the molecular and structure determinants by which these proteins stabilize microtubules remain under debate. We show here that a short protein fragment from MAP6-N, which encompasses its Mn1 and Mn2 modules (MAP6(90-177)), recapitulates the function of the full-length MAP6-N protein toward microtubules, i.e. its ability to stabilize microtubules in vitro and in cultured cells in ice-cold conditions or in the presence of nocodazole. We further show for the first time, using biochemical assays and NMR spectroscopy, that these effects result from the binding of MAP6(90-177) to microtubules with a 1:1 MAP6(90-177):tubulin heterodimer stoichiometry. NMR data demonstrate that the binding of MAP6(90-177) to microtubules involve its two Mn modules but that a single one is also able to interact with microtubules in a closely similar manner. This suggests that the Mn modules represent each a full microtubule binding domain and that MAP6 proteins may stabilize microtubules by bridging tubulin heterodimers from adjacent protofilaments or within a protofilament. Finally, we demonstrate that Ca(2+)-calmodulin competes with microtubules for MAP6(90-177) binding and that the binding mode of MAP6(90-177) to microtubules and Ca(2+)-calmodulin involves a common stretch of amino acid residues on the MAP6(90-177) side. This result accounts for the regulation of microtubule stability in cold condition by Ca(2+)-calmodulin."xsd:string |
| http://purl.uniprot.org/citations/23831686 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m113.457267"xsd:string |
| http://purl.uniprot.org/citations/23831686 | http://purl.uniprot.org/core/author | "Gans P."xsd:string |
| http://purl.uniprot.org/citations/23831686 | http://purl.uniprot.org/core/author | "Bosc C."xsd:string |
| http://purl.uniprot.org/citations/23831686 | http://purl.uniprot.org/core/author | "Andrieux A."xsd:string |
| http://purl.uniprot.org/citations/23831686 | http://purl.uniprot.org/core/author | "Clement M.J."xsd:string |
| http://purl.uniprot.org/citations/23831686 | http://purl.uniprot.org/core/author | "Curmi P.A."xsd:string |
| http://purl.uniprot.org/citations/23831686 | http://purl.uniprot.org/core/author | "Savarin P."xsd:string |
| http://purl.uniprot.org/citations/23831686 | http://purl.uniprot.org/core/author | "Lefevre J."xsd:string |
| http://purl.uniprot.org/citations/23831686 | http://purl.uniprot.org/core/author | "Hamon L."xsd:string |
| http://purl.uniprot.org/citations/23831686 | http://purl.uniprot.org/core/author | "David M.O."xsd:string |
| http://purl.uniprot.org/citations/23831686 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23831686 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/23831686 | http://purl.uniprot.org/core/pages | "24910-24922"xsd:string |
| http://purl.uniprot.org/citations/23831686 | http://purl.uniprot.org/core/title | "Structural basis for the association of MAP6 protein with microtubules and its regulation by calmodulin."xsd:string |
| http://purl.uniprot.org/citations/23831686 | http://purl.uniprot.org/core/volume | "288"xsd:string |
| http://purl.uniprot.org/citations/23831686 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23831686 |
| http://purl.uniprot.org/citations/23831686 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/23831686 |
| http://purl.uniprot.org/uniprot/#_A6I6G6-mappedCitation-23831686 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23831686 |
| http://purl.uniprot.org/uniprot/#_B4DJ51-mappedCitation-23831686 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23831686 |
| http://purl.uniprot.org/uniprot/#_Q63560-mappedCitation-23831686 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23831686 |
| http://purl.uniprot.org/uniprot/#_P0DP23-mappedCitation-23831686 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23831686 |
| http://purl.uniprot.org/uniprot/#_P0DP24-mappedCitation-23831686 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23831686 |
| http://purl.uniprot.org/uniprot/#_P0DP25-mappedCitation-23831686 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23831686 |