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http://purl.uniprot.org/citations/23843614http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/23843614http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/23843614http://www.w3.org/2000/01/rdf-schema#comment"Astrocytes exhibit a complex, branched morphology, allowing them to functionally interact with numerous blood vessels, neighboring glial processes and neuronal elements, including synapses. They also respond to central nervous system (CNS) injury by a process known as astrogliosis, which involves morphological changes, including cell body hypertrophy and thickening of major processes. Following severe injury, astrocytes exhibit drastically reduced morphological complexity and collectively form a glial scar. The mechanistic details behind these morphological changes are unknown. Here, we investigate the regulation of the actin-nucleating Arp2/3 complex in controlling dynamic changes in astrocyte morphology. In contrast to other cell types, Arp2/3 inhibition drives the rapid expansion of astrocyte cell bodies and major processes. This intervention results in a reduced morphological complexity of astrocytes in both dissociated culture and in brain slices. We show that this expansion requires functional myosin II downstream of ROCK and RhoA. Knockdown of the Arp2/3 subunit Arp3 or the Arp2/3 activator N-WASP by siRNA also results in cell body expansion and reduced morphological complexity, whereas depleting WAVE2 specifically reduces the branching complexity of astrocyte processes. By contrast, knockdown of the Arp2/3 inhibitor PICK1 increases astrocyte branching complexity. Furthermore, astrocyte expansion induced by ischemic conditions is delayed by PICK1 knockdown or N-WASP overexpression. Our findings identify a new morphological outcome for Arp2/3 activation in restricting rather than promoting outwards movement of the plasma membrane in astrocytes. The Arp2/3 regulators PICK1, and N-WASP and WAVE2 function antagonistically to control the complexity of astrocyte branched morphology, and this mechanism underlies the morphological changes seen in astrocytes during their response to pathological insult."xsd:string
http://purl.uniprot.org/citations/23843614http://purl.org/dc/terms/identifier"doi:10.1242/jcs.125146"xsd:string
http://purl.uniprot.org/citations/23843614http://purl.org/dc/terms/identifier"doi:10.1242/jcs.125146"xsd:string
http://purl.uniprot.org/citations/23843614http://purl.uniprot.org/core/author"Hanley J.G."xsd:string
http://purl.uniprot.org/citations/23843614http://purl.uniprot.org/core/author"Hanley J.G."xsd:string
http://purl.uniprot.org/citations/23843614http://purl.uniprot.org/core/author"Murk K."xsd:string
http://purl.uniprot.org/citations/23843614http://purl.uniprot.org/core/author"Murk K."xsd:string
http://purl.uniprot.org/citations/23843614http://purl.uniprot.org/core/author"Banks P."xsd:string
http://purl.uniprot.org/citations/23843614http://purl.uniprot.org/core/author"Banks P."xsd:string
http://purl.uniprot.org/citations/23843614http://purl.uniprot.org/core/author"Blanco Suarez E.M."xsd:string
http://purl.uniprot.org/citations/23843614http://purl.uniprot.org/core/author"Blanco Suarez E.M."xsd:string
http://purl.uniprot.org/citations/23843614http://purl.uniprot.org/core/author"Cockbill L.M."xsd:string
http://purl.uniprot.org/citations/23843614http://purl.uniprot.org/core/author"Cockbill L.M."xsd:string
http://purl.uniprot.org/citations/23843614http://purl.uniprot.org/core/date"2013"xsd:gYear
http://purl.uniprot.org/citations/23843614http://purl.uniprot.org/core/date"2013"xsd:gYear
http://purl.uniprot.org/citations/23843614http://purl.uniprot.org/core/name"J. Cell Sci."xsd:string
http://purl.uniprot.org/citations/23843614http://purl.uniprot.org/core/name"J. Cell Sci."xsd:string
http://purl.uniprot.org/citations/23843614http://purl.uniprot.org/core/pages"3873-3883"xsd:string
http://purl.uniprot.org/citations/23843614http://purl.uniprot.org/core/pages"3873-3883"xsd:string
http://purl.uniprot.org/citations/23843614http://purl.uniprot.org/core/title"The antagonistic modulation of Arp2/3 activity by N-WASP, WAVE2 and PICK1 defines dynamic changes in astrocyte morphology."xsd:string
http://purl.uniprot.org/citations/23843614http://purl.uniprot.org/core/title"The antagonistic modulation of Arp2/3 activity by N-WASP, WAVE2 and PICK1 defines dynamic changes in astrocyte morphology."xsd:string
http://purl.uniprot.org/citations/23843614http://purl.uniprot.org/core/volume"126"xsd:string
http://purl.uniprot.org/citations/23843614http://purl.uniprot.org/core/volume"126"xsd:string
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