| http://purl.uniprot.org/citations/23860929 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23860929 | http://www.w3.org/2000/01/rdf-schema#comment | "Ductal carcinoma in situ (DCIS) of the breast is difficult to remove completely during surgery as it is not palpable and can therefore require re-excision. Real-time visualization of DCIS using near-infrared fluorescent probes could help the surgeon during surgery as well as the pathologist post-operatively to distinguish the tumor from healthy tissue. As hypoxia-induced necrosis is a common phenomenon in DCIS, we investigated the molecular imaging of DCIS using a fluorescent antibody targeting a hypoxia marker, carbonic anhydrase IX (CAIX), in a preclinical mouse model. A monoclonal antibody against human CAIX was fluorescently labeled with the near-infrared dye IRDye800CW and characterized in vitro. An in vivo study was performed in SCID/Beige mice that were orthotopically transplanted with human breast cancer cells mimicking human DCIS (MCF10DCIS) and MCF10DCIS stably expressing CAIX. A clinically approved fluorescence imaging system was used to monitor probe uptake and to determine tumor-to-normal tissue ratios (TNR). Mean in vivo TNR of CAIX-transduced (CAIX+) tumors was 7.5 ± 0.5. Mean in vivo TNR of DCIS tumors with hypoxic areas reached a plateau level at 48 h after injection of 2.1 ± 0.1 (mean ± SEM) compared to 1.7 ± 0.1 in DCIS without hypoxic areas. Mean intra-operative TNR of DCIS tumors with necrotic regions was higher than that of DCIS tumors without necrotic regions 96 h after injection-2.9 ± 0.1 and 1.5 ± 0.1, respectively-while the TNR of CAIX+ tumors was 11.2 ± 1.0. Specific tumor uptake of MabCAIX-IRDye800CW was confirmed by a biodistribution assay, and immunofluorescence imaging on tumor sections showed specific uptake in hypoxic tumor regions, with higher contrast than conventional chromagen-based immunohistochemistry. Molecular fluorescence imaging with MabCAIX-IRDye800CW can be successfully used to detect hypoxic DCIS before and during surgery to facilitate radical resection. Furthermore, it allows for sensitive CAIX-specific immunofluorescence microscopy of tumor sections, thereby introducing the concept of molecular fluorescence pathology."xsd:string |
| http://purl.uniprot.org/citations/23860929 | http://purl.org/dc/terms/identifier | "doi:10.1007/s10549-013-2635-6"xsd:string |
| http://purl.uniprot.org/citations/23860929 | http://purl.uniprot.org/core/author | "Oliveira S."xsd:string |
| http://purl.uniprot.org/citations/23860929 | http://purl.uniprot.org/core/author | "Adams A."xsd:string |
| http://purl.uniprot.org/citations/23860929 | http://purl.uniprot.org/core/author | "Vermeulen J.F."xsd:string |
| http://purl.uniprot.org/citations/23860929 | http://purl.uniprot.org/core/author | "van Diest P.J."xsd:string |
| http://purl.uniprot.org/citations/23860929 | http://purl.uniprot.org/core/author | "van der Wall E."xsd:string |
| http://purl.uniprot.org/citations/23860929 | http://purl.uniprot.org/core/author | "van Bergen En Henegouwen P.M."xsd:string |
| http://purl.uniprot.org/citations/23860929 | http://purl.uniprot.org/core/author | "Mali W.P."xsd:string |
| http://purl.uniprot.org/citations/23860929 | http://purl.uniprot.org/core/author | "van Brussel A.S."xsd:string |
| http://purl.uniprot.org/citations/23860929 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23860929 | http://purl.uniprot.org/core/name | "Breast Cancer Res Treat"xsd:string |
| http://purl.uniprot.org/citations/23860929 | http://purl.uniprot.org/core/pages | "263-272"xsd:string |
| http://purl.uniprot.org/citations/23860929 | http://purl.uniprot.org/core/title | "Molecular imaging with a fluorescent antibody targeting carbonic anhydrase IX can successfully detect hypoxic ductal carcinoma in situ of the breast."xsd:string |
| http://purl.uniprot.org/citations/23860929 | http://purl.uniprot.org/core/volume | "140"xsd:string |
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