| http://purl.uniprot.org/citations/23892456 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23892456 | http://www.w3.org/2000/01/rdf-schema#comment | "Embryonic stem (ES) cell self-renewal efficiency is determined by the Nanog protein level. However, the protein partners of Nanog that function to direct self-renewal are unclear. Here, we identify a Nanog interactome of over 130 proteins including transcription factors, chromatin modifying complexes, phosphorylation and ubiquitination enzymes, basal transcriptional machinery members, and RNA processing factors. Sox2 was identified as a robust interacting partner of Nanog. The purified Nanog-Sox2 complex identified a DNA recognition sequence present in multiple overlapping Nanog/Sox2 ChIP-Seq data sets. The Nanog tryptophan repeat region is necessary and sufficient for interaction with Sox2, with tryptophan residues required. In Sox2, tyrosine to alanine mutations within a triple-repeat motif (S X T/S Y) abrogates the Nanog-Sox2 interaction, alters expression of genes associated with the Nanog-Sox2 cognate sequence, and reduces the ability of Sox2 to rescue ES cell differentiation induced by endogenous Sox2 deletion. Substitution of the tyrosines with phenylalanine rescues both the Sox2-Nanog interaction and efficient self-renewal. These results suggest that aromatic stacking of Nanog tryptophans and Sox2 tyrosines mediates an interaction central to ES cell self-renewal."xsd:string |
| http://purl.uniprot.org/citations/23892456 | http://purl.org/dc/terms/identifier | "doi:10.1038/emboj.2013.161"xsd:string |
| http://purl.uniprot.org/citations/23892456 | http://purl.uniprot.org/core/author | "Demmers J."xsd:string |
| http://purl.uniprot.org/citations/23892456 | http://purl.uniprot.org/core/author | "Mullin N.P."xsd:string |
| http://purl.uniprot.org/citations/23892456 | http://purl.uniprot.org/core/author | "Poot R.A."xsd:string |
| http://purl.uniprot.org/citations/23892456 | http://purl.uniprot.org/core/author | "Bezstarosti K."xsd:string |
| http://purl.uniprot.org/citations/23892456 | http://purl.uniprot.org/core/author | "Favaro R."xsd:string |
| http://purl.uniprot.org/citations/23892456 | http://purl.uniprot.org/core/author | "Tomlinson S.R."xsd:string |
| http://purl.uniprot.org/citations/23892456 | http://purl.uniprot.org/core/author | "Chambers I."xsd:string |
| http://purl.uniprot.org/citations/23892456 | http://purl.uniprot.org/core/author | "Colby D."xsd:string |
| http://purl.uniprot.org/citations/23892456 | http://purl.uniprot.org/core/author | "Halbritter F."xsd:string |
| http://purl.uniprot.org/citations/23892456 | http://purl.uniprot.org/core/author | "Felker A."xsd:string |
| http://purl.uniprot.org/citations/23892456 | http://purl.uniprot.org/core/author | "Gagliardi A."xsd:string |
| http://purl.uniprot.org/citations/23892456 | http://purl.uniprot.org/core/author | "Nicolis S.K."xsd:string |
| http://purl.uniprot.org/citations/23892456 | http://purl.uniprot.org/core/author | "Kousa A.I."xsd:string |
| http://purl.uniprot.org/citations/23892456 | http://purl.uniprot.org/core/author | "Weiss J.T."xsd:string |
| http://purl.uniprot.org/citations/23892456 | http://purl.uniprot.org/core/author | "Ying Tan Z."xsd:string |
| http://purl.uniprot.org/citations/23892456 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23892456 | http://purl.uniprot.org/core/name | "EMBO J"xsd:string |
| http://purl.uniprot.org/citations/23892456 | http://purl.uniprot.org/core/pages | "2231-2247"xsd:string |
| http://purl.uniprot.org/citations/23892456 | http://purl.uniprot.org/core/title | "A direct physical interaction between Nanog and Sox2 regulates embryonic stem cell self-renewal."xsd:string |
| http://purl.uniprot.org/citations/23892456 | http://purl.uniprot.org/core/volume | "32"xsd:string |
| http://purl.uniprot.org/citations/23892456 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23892456 |
| http://purl.uniprot.org/citations/23892456 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/23892456 |