| http://purl.uniprot.org/citations/2391346 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2391346 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2391346 | http://www.w3.org/2000/01/rdf-schema#comment | "Two forms of cytochrome P-450 (P-450) from liver microsomes of hamsters treated with 2,3,4,7,8-pentachlorodibenzofuran (PenCDF), which possesses the potent acute toxicity and 3-methylcholanthrene (MC)-type inducing ability of liver microsomal monooxygenases in animals, were purified and characterized. These P-450 forms, designated as hamster P-450H and hamster P-450L, had the molecular masses of 52 and 50 kDa, respectively, and showed the absorption maximum of CO-reduced difference spectra at 446 nm. The absolute spectra of their oxidized forms indicated that hamster P-450H was in high-spin state and hamster P-450L was in low-spin state. A part of PenCDF injected into hamster was tightly bound to purified hamster P-450H at a ratio of 0.107 nmol PenCDF/nmol P-450. In a reconstituted system, both hamster P-450H and hamster P-450L showed relatively low catalytic activities for 3-hydroxylation of benzo[a]pyrene and O-deethylations of both 7-ethoxyresorufin and 7-ethoxycoumarin, while they both catalyzed 7 alpha- and 2 alpha-hydroxylations of testosterone effectively to a similar extent. Addition of cytochrome b5-to a reconstituted system accelerated the formation of 7 alpha-hydroxytestosterone 5.3-fold with hamster P-450L and 2.2-fold with hamster P-450H. In addition, hamster P-450H catalyzed estradiol 2-hydroxylation at a high rate but hamster P-450L did not.(ABSTRACT TRUNCATED AT 250 WORDS)"xsd:string |
| http://purl.uniprot.org/citations/2391346 | http://purl.org/dc/terms/identifier | "doi:10.1093/oxfordjournals.jbchem.a123133"xsd:string |
| http://purl.uniprot.org/citations/2391346 | http://purl.org/dc/terms/identifier | "doi:10.1093/oxfordjournals.jbchem.a123133"xsd:string |
| http://purl.uniprot.org/citations/2391346 | http://purl.uniprot.org/core/author | "Nakashima H."xsd:string |
| http://purl.uniprot.org/citations/2391346 | http://purl.uniprot.org/core/author | "Nakashima H."xsd:string |
| http://purl.uniprot.org/citations/2391346 | http://purl.uniprot.org/core/author | "Yoshimura H."xsd:string |
| http://purl.uniprot.org/citations/2391346 | http://purl.uniprot.org/core/author | "Yoshimura H."xsd:string |
| http://purl.uniprot.org/citations/2391346 | http://purl.uniprot.org/core/author | "Ariyoshi N."xsd:string |
| http://purl.uniprot.org/citations/2391346 | http://purl.uniprot.org/core/author | "Ariyoshi N."xsd:string |
| http://purl.uniprot.org/citations/2391346 | http://purl.uniprot.org/core/author | "Koga N."xsd:string |
| http://purl.uniprot.org/citations/2391346 | http://purl.uniprot.org/core/author | "Koga N."xsd:string |
| http://purl.uniprot.org/citations/2391346 | http://purl.uniprot.org/core/date | "1990"xsd:gYear |
| http://purl.uniprot.org/citations/2391346 | http://purl.uniprot.org/core/date | "1990"xsd:gYear |
| http://purl.uniprot.org/citations/2391346 | http://purl.uniprot.org/core/name | "J. Biochem."xsd:string |
| http://purl.uniprot.org/citations/2391346 | http://purl.uniprot.org/core/name | "J. Biochem."xsd:string |
| http://purl.uniprot.org/citations/2391346 | http://purl.uniprot.org/core/pages | "826-833"xsd:string |
| http://purl.uniprot.org/citations/2391346 | http://purl.uniprot.org/core/pages | "826-833"xsd:string |
| http://purl.uniprot.org/citations/2391346 | http://purl.uniprot.org/core/title | "Purification and characterization of two forms of 2,3,4,7,8-pentachlorodibenzofuran-inducible cytochrome P-450 in hamster liver."xsd:string |
| http://purl.uniprot.org/citations/2391346 | http://purl.uniprot.org/core/title | "Purification and characterization of two forms of 2,3,4,7,8-pentachlorodibenzofuran-inducible cytochrome P-450 in hamster liver."xsd:string |
| http://purl.uniprot.org/citations/2391346 | http://purl.uniprot.org/core/volume | "107"xsd:string |
| http://purl.uniprot.org/citations/2391346 | http://purl.uniprot.org/core/volume | "107"xsd:string |
| http://purl.uniprot.org/citations/2391346 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/2391346 |
| http://purl.uniprot.org/citations/2391346 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/2391346 |