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http://purl.uniprot.org/citations/23918784http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/23918784http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/23918784http://www.w3.org/2000/01/rdf-schema#comment"Bacillus thuringiensis produces toxins that target invertebrates, including Caenorhabditis elegans. Virulence of Bacillus strains is often highly specific, such that B. thuringiensis strain DB27 is highly pathogenic to C. elegans but shows no virulence for another model nematode, Pristionchus pacificus. To uncover the underlying mechanisms of the differential responses of the two nematodes to B. thuringiensis DB27 and to reveal the C. elegans defense mechanisms against this pathogen, we conducted a genetic screen for C. elegans mutants resistant to B. thuringiensis DB27. Here, we describe a B. thuringiensis DB27-resistant C. elegans mutant that is identical to nasp-1, which encodes the C. elegans homolog of the nuclear-autoantigenic-sperm protein. Gene expression analysis indicated a substantial overlap between the genes downregulated in the nasp-1 mutant and targets of C. elegans dcr-1/Dicer, suggesting that dcr-1 is repressed in nasp-1 mutants, which was confirmed by quantitative PCR. Consistent with this, the nasp-1 mutant exhibits RNA interference (RNAi) deficiency and reduced longevity similar to those of a dcr-1 mutant. Building on these surprising findings, we further explored a potential role for dcr-1 in C. elegans innate immunity. We show that dcr-1 mutant alleles deficient in microRNA (miRNA) processing, but not those deficient only in RNAi, are resistant to B. thuringiensis DB27. Furthermore, dcr-1 overexpression rescues the nasp-1 mutant's resistance, suggesting that repression of dcr-1 determines the nasp-1 mutant's resistance. Additionally, we identified the collagen-encoding gene col-92 as one of the downstream effectors of nasp-1 that play an important role in resistance to DB27. Taken together, these results uncover a previously unknown role for DCR-1/Dicer in C. elegans antibacterial immunity that is largely associated with miRNA processing."xsd:string
http://purl.uniprot.org/citations/23918784http://purl.org/dc/terms/identifier"doi:10.1128/iai.00700-13"xsd:string
http://purl.uniprot.org/citations/23918784http://purl.org/dc/terms/identifier"doi:10.1128/iai.00700-13"xsd:string
http://purl.uniprot.org/citations/23918784http://purl.uniprot.org/core/author"Sinha A."xsd:string
http://purl.uniprot.org/citations/23918784http://purl.uniprot.org/core/author"Sinha A."xsd:string
http://purl.uniprot.org/citations/23918784http://purl.uniprot.org/core/author"Iatsenko I."xsd:string
http://purl.uniprot.org/citations/23918784http://purl.uniprot.org/core/author"Iatsenko I."xsd:string
http://purl.uniprot.org/citations/23918784http://purl.uniprot.org/core/author"Roedelsperger C."xsd:string
http://purl.uniprot.org/citations/23918784http://purl.uniprot.org/core/author"Roedelsperger C."xsd:string
http://purl.uniprot.org/citations/23918784http://purl.uniprot.org/core/author"Sommer R.J."xsd:string
http://purl.uniprot.org/citations/23918784http://purl.uniprot.org/core/author"Sommer R.J."xsd:string
http://purl.uniprot.org/citations/23918784http://purl.uniprot.org/core/date"2013"xsd:gYear
http://purl.uniprot.org/citations/23918784http://purl.uniprot.org/core/date"2013"xsd:gYear
http://purl.uniprot.org/citations/23918784http://purl.uniprot.org/core/name"Infect. Immun."xsd:string
http://purl.uniprot.org/citations/23918784http://purl.uniprot.org/core/name"Infect. Immun."xsd:string
http://purl.uniprot.org/citations/23918784http://purl.uniprot.org/core/pages"3942-3957"xsd:string
http://purl.uniprot.org/citations/23918784http://purl.uniprot.org/core/pages"3942-3957"xsd:string
http://purl.uniprot.org/citations/23918784http://purl.uniprot.org/core/title"New role for DCR-1/dicer in Caenorhabditis elegans innate immunity against the highly virulent bacterium Bacillus thuringiensis DB27."xsd:string
http://purl.uniprot.org/citations/23918784http://purl.uniprot.org/core/title"New role for DCR-1/dicer in Caenorhabditis elegans innate immunity against the highly virulent bacterium Bacillus thuringiensis DB27."xsd:string
http://purl.uniprot.org/citations/23918784http://purl.uniprot.org/core/volume"81"xsd:string
http://purl.uniprot.org/citations/23918784http://purl.uniprot.org/core/volume"81"xsd:string
http://purl.uniprot.org/citations/23918784http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/23918784
http://purl.uniprot.org/citations/23918784http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/23918784
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