| http://purl.uniprot.org/citations/23918979 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23918979 | http://www.w3.org/2000/01/rdf-schema#comment | "In its resting state, Rho GDP-dissociation inhibitor (RhoGDI) α forms a soluble cytoplasmic heterodimer with the GDP-bound form of Rac. Upon stimulation, the dissociation of RhoGDIα from the RhoGDIα-Rac complex is a mandatory step for Rac activation; however, this mechanism is poorly understood. In this study, we examined how the cytoplasm/membrane cycles of the RhoGDI-Rac complex are regulated, as well as where RhoGDI dissociates from the RhoGDI-Rac complex, during FcγR-mediated phagocytosis. The negatively charged and flexible N terminus (25 residues) of RhoGDIα, particularly its second negative amino acid cluster possessing five negatively charged amino acids, was a pivotal regulator in the cytoplasm/membrane cycles of the RhoGDI-Rac complex. We also found that RhoGDIα translocated to the phagosomes as a RhoGDIα-Rac1 complex, and this translocation was mediated by an interaction between the polybasic motif in the C terminus of Rac1 and anionic phospholipids produced on phagosomes, such as phosphatidic acid, that is, by a phagosome-targeting mechanism of Rac1. Thus, we demonstrated that the targeting/accumulation of the RhoGDIα-Rac1 complex to phagosomes is regulated by a balance between three factors: 1) the negatively charged and flexible N-terminal of RhoGDIα, 2) the binding affinity of RhoGDIα for Rac1, and 3) anionic phospholipids produced on phagosomes. Moreover, we demonstrated that the mechanism of targeting/accumulation of the RhoGDIα-Rac1 complex is also applicable for the RhoGDIβ-Rac1 complex."xsd:string |
| http://purl.uniprot.org/citations/23918979 | http://purl.org/dc/terms/identifier | "doi:10.4049/jimmunol.1300209"xsd:string |
| http://purl.uniprot.org/citations/23918979 | http://purl.uniprot.org/core/author | "Kobayashi T."xsd:string |
| http://purl.uniprot.org/citations/23918979 | http://purl.uniprot.org/core/author | "Nakamura T."xsd:string |
| http://purl.uniprot.org/citations/23918979 | http://purl.uniprot.org/core/author | "Saito N."xsd:string |
| http://purl.uniprot.org/citations/23918979 | http://purl.uniprot.org/core/author | "Hamada T."xsd:string |
| http://purl.uniprot.org/citations/23918979 | http://purl.uniprot.org/core/author | "Ueyama T."xsd:string |
| http://purl.uniprot.org/citations/23918979 | http://purl.uniprot.org/core/author | "Sakaguchi H."xsd:string |
| http://purl.uniprot.org/citations/23918979 | http://purl.uniprot.org/core/author | "Son J."xsd:string |
| http://purl.uniprot.org/citations/23918979 | http://purl.uniprot.org/core/author | "Shirafuji T."xsd:string |
| http://purl.uniprot.org/citations/23918979 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23918979 | http://purl.uniprot.org/core/name | "J Immunol"xsd:string |
| http://purl.uniprot.org/citations/23918979 | http://purl.uniprot.org/core/pages | "2560-2569"xsd:string |
| http://purl.uniprot.org/citations/23918979 | http://purl.uniprot.org/core/title | "Negative charges in the flexible N-terminal domain of Rho GDP-dissociation inhibitors (RhoGDIs) regulate the targeting of the RhoGDI-Rac1 complex to membranes."xsd:string |
| http://purl.uniprot.org/citations/23918979 | http://purl.uniprot.org/core/volume | "191"xsd:string |
| http://purl.uniprot.org/citations/23918979 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23918979 |
| http://purl.uniprot.org/citations/23918979 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/23918979 |
| http://purl.uniprot.org/uniprot/#_A0A0S2Z3D9-mappedCitation-23918979 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23918979 |
| http://purl.uniprot.org/uniprot/#_P52565-mappedCitation-23918979 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23918979 |
| http://purl.uniprot.org/uniprot/#_J3QQX2-mappedCitation-23918979 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23918979 |
| http://purl.uniprot.org/uniprot/#_J3QW41-mappedCitation-23918979 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23918979 |
| http://purl.uniprot.org/uniprot/#_Q8BPG5-mappedCitation-23918979 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23918979 |
| http://purl.uniprot.org/uniprot/#_K7Q7T7-mappedCitation-23918979 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23918979 |
| http://purl.uniprot.org/uniprot/#_P63001-mappedCitation-23918979 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23918979 |