| http://purl.uniprot.org/citations/23926355 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23926355 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23926355 | http://www.w3.org/2000/01/rdf-schema#comment | "The human endogenous retrovirus family HERV-K(HML-2) Rec protein is an RNA transport factor that enhances nuclear export of intron-containing retroviral transcripts. Using the yeast two-hybrid approach, we have newly identified human Staufen-1 as a Rec-interacting protein. The interaction was confirmed by coimmunoprecipitation experiments, and the relevant site in Staufen-1 has been mapped to double-stranded RNA binding domain 4 (RBD4). Staufen-1 is in several aspects functionally related to retroviral RNA transport proteins. It binds mRNAs and targets its ribonuclear cargo to polysomes for efficient translation. We observed an accumulation of Staufen-1 in the nucleus of Rec-expressing cells and colocalization in the nucleoli as well as in the cytoplasm. Overexpression of Staufen-1 resulted in a 5-fold enhancement in nuclear export and/or translation of unspliced HERV-K(HML-2) viral RNAs in the presence of Rec and its Rec-responsive element (RcRE) binding site together with a clear increase in virus production. Staufen-1 was previously shown to interact with the Gag protein of HIV-1, promoting Gag oligomerization and RNA encapsidation. We demonstrate here that Staufen-1 also binds to the Gag protein of HERV-K(HML-2). Under stress conditions, Rec colocalizes with Staufen-1 in stress granules in cells that express viral RNA but not in mRNA-decay-related processing bodies. Our results suggest a new role for Staufen-1 as a cellular Rec and HERV-K(HML-2) Gag cofactor."xsd:string |
| http://purl.uniprot.org/citations/23926355 | http://purl.org/dc/terms/identifier | "doi:10.1128/jvi.03031-12"xsd:string |
| http://purl.uniprot.org/citations/23926355 | http://purl.org/dc/terms/identifier | "doi:10.1128/jvi.03031-12"xsd:string |
| http://purl.uniprot.org/citations/23926355 | http://purl.uniprot.org/core/author | "Kurth R."xsd:string |
| http://purl.uniprot.org/citations/23926355 | http://purl.uniprot.org/core/author | "Kurth R."xsd:string |
| http://purl.uniprot.org/citations/23926355 | http://purl.uniprot.org/core/author | "Bannert N."xsd:string |
| http://purl.uniprot.org/citations/23926355 | http://purl.uniprot.org/core/author | "Bannert N."xsd:string |
| http://purl.uniprot.org/citations/23926355 | http://purl.uniprot.org/core/author | "Hohn O."xsd:string |
| http://purl.uniprot.org/citations/23926355 | http://purl.uniprot.org/core/author | "Hohn O."xsd:string |
| http://purl.uniprot.org/citations/23926355 | http://purl.uniprot.org/core/author | "Hanke K."xsd:string |
| http://purl.uniprot.org/citations/23926355 | http://purl.uniprot.org/core/author | "Hanke K."xsd:string |
| http://purl.uniprot.org/citations/23926355 | http://purl.uniprot.org/core/author | "Fiddeke K."xsd:string |
| http://purl.uniprot.org/citations/23926355 | http://purl.uniprot.org/core/author | "Fiddeke K."xsd:string |
| http://purl.uniprot.org/citations/23926355 | http://purl.uniprot.org/core/author | "Liedgens L."xsd:string |
| http://purl.uniprot.org/citations/23926355 | http://purl.uniprot.org/core/author | "Liedgens L."xsd:string |
| http://purl.uniprot.org/citations/23926355 | http://purl.uniprot.org/core/author | "Wamara J."xsd:string |
| http://purl.uniprot.org/citations/23926355 | http://purl.uniprot.org/core/author | "Wamara J."xsd:string |
| http://purl.uniprot.org/citations/23926355 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23926355 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23926355 | http://purl.uniprot.org/core/name | "J. Virol."xsd:string |
| http://purl.uniprot.org/citations/23926355 | http://purl.uniprot.org/core/name | "J. Virol."xsd:string |
| http://purl.uniprot.org/citations/23926355 | http://purl.uniprot.org/core/pages | "11019-11030"xsd:string |
| http://purl.uniprot.org/citations/23926355 | http://purl.uniprot.org/core/pages | "11019-11030"xsd:string |