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| http://purl.uniprot.org/citations/23935538 | http://www.w3.org/2000/01/rdf-schema#comment | "8-oxoG is one of the most common and mutagenic DNA base lesions caused by oxidative damage. However, it has not been possible to study the replication of a known 8-oxoG base in vivo in order to determine the accuracy of its replication, the influence of various components on that accuracy, and the extent to which an 8-oxoG might present a barrier to replication. We have been able to place a single 8-oxoG into the Saccharomyces cerevisiae chromosome in a defined location using single-strand oligonucleotide transformation and to study its replication in a fully normal chromosome context. During replication, 8-oxoG is recognized as a lesion and triggers a switch to translesion synthesis by Pol η, which replicates 8-oxoG with an accuracy (insertion of a C opposite the 8-oxoG) of approximately 94%. In the absence of Pol η, template switching to the newly synthesized sister chromatid is observed at least one third of the time; replication of the 8-oxoG in the absence of Pol η is less than 40% accurate. The mismatch repair (MMR) system plays an important role in 8-oxoG replication. Template switching is blocked by MMR and replication accuracy even in the absence of Pol η is approximately 95% when MMR is active. These findings indicate that in light of the overlapping mechanisms by which errors in 8-oxoG replication can be avoided in the cell, the mutagenic threat of 8-oxoG is due more to its abundance than the effect of a single lesion. In addition, the methods used here should be applicable to the study of any lesion that can be stably incorporated into synthetic oligonucleotides."xsd:string |
| http://purl.uniprot.org/citations/23935538 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pgen.1003682"xsd:string |
| http://purl.uniprot.org/citations/23935538 | http://purl.uniprot.org/core/author | "Crouse G.F."xsd:string |
| http://purl.uniprot.org/citations/23935538 | http://purl.uniprot.org/core/author | "Rodriguez G.P."xsd:string |
| http://purl.uniprot.org/citations/23935538 | http://purl.uniprot.org/core/author | "Song J.B."xsd:string |
| http://purl.uniprot.org/citations/23935538 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23935538 | http://purl.uniprot.org/core/name | "PLoS Genet"xsd:string |
| http://purl.uniprot.org/citations/23935538 | http://purl.uniprot.org/core/pages | "e1003682"xsd:string |
| http://purl.uniprot.org/citations/23935538 | http://purl.uniprot.org/core/title | "In vivo bypass of 8-oxodG."xsd:string |
| http://purl.uniprot.org/citations/23935538 | http://purl.uniprot.org/core/volume | "9"xsd:string |
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