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http://purl.uniprot.org/citations/23948415http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/23948415http://www.w3.org/2000/01/rdf-schema#comment"

Objective

To study the expression of caspase recruitment domain family member 18 (CARD18) in apoptin-induced apoptosis of gastric cancer cells and its role in the development of gastric cancer.

Methods

After gastric cancer cells were transfected with apoptin, differentially expressed proteins between the apoptin-expression SGC7901 group and the control group were seperated using two-dimensional gel electrophoresis and identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and Mascot database (http: //www.matrixscience.com/). The expression level and location of CARD18 in 56 cases of gastric adenocarcinoma and adjacent cancer-free tissues were respectively detected by RT-PCR, Western blotting and immunohistochemistry to analyze the relationship between CARD18 and the clinical pathological characteristics.

Results

RT-PCR and Western blotting showed that the level of CARD18 mRNA and protein was down-regulated the most significantly after apoptin treatment. The expression of CARD18 in gastric adenocarcinoma tissues was significantly higher than that in adjacent cancer-free tissues (P<0.05), and it was proved that the CARD18 expression was related to the gastric cancer lymph node metastasis and TNM stage (P<0.05). CONCLUSION CARD18 may be both a promising marker for prognosis and a target protein for treatment of gastric cancer."xsd:string
http://purl.uniprot.org/citations/23948415http://purl.uniprot.org/core/author"Liu S."xsd:string
http://purl.uniprot.org/citations/23948415http://purl.uniprot.org/core/author"Xiang Z."xsd:string
http://purl.uniprot.org/citations/23948415http://purl.uniprot.org/core/author"Tan C."xsd:string
http://purl.uniprot.org/citations/23948415http://purl.uniprot.org/core/date"2013"xsd:gYear
http://purl.uniprot.org/citations/23948415http://purl.uniprot.org/core/name"Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi"xsd:string
http://purl.uniprot.org/citations/23948415http://purl.uniprot.org/core/pages"858-861"xsd:string
http://purl.uniprot.org/citations/23948415http://purl.uniprot.org/core/title"[The expression of CARD18 in apoptin-transfected gastric cancer cells and gastric adenocarcinoma tissues]."xsd:string
http://purl.uniprot.org/citations/23948415http://purl.uniprot.org/core/volume"29"xsd:string
http://purl.uniprot.org/citations/23948415http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/23948415
http://purl.uniprot.org/citations/23948415http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/23948415
http://purl.uniprot.org/uniprot/#_P57730-mappedCitation-23948415http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/23948415
http://purl.uniprot.org/uniprot/#_G5EA35-mappedCitation-23948415http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/23948415
http://purl.uniprot.org/uniprot/P57730http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/23948415
http://purl.uniprot.org/uniprot/G5EA35http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/23948415