| http://purl.uniprot.org/citations/23951055 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23951055 | http://www.w3.org/2000/01/rdf-schema#comment | "Non-muscle myosin II is stimulated by monophosphorylation of its regulatory light chain (MRLC) at Ser19 (1P-MRLC). MRLC diphosphorylation at Thr18/Ser19 (2P-MRLC) further enhances the ATPase activity of myosin II. Phosphorylated MRLCs localize to the contractile ring and regulate cytokinesis as subunits of activated myosin II. Recently, we reported that 2P-MRLC, but not 1P-MRLC, localizes to the midzone independently of myosin II heavy chain during cytokinesis in cultured mammalian cells. However, the mechanism underlying the distinct localization of 1P- and 2P-MRLC during cytokinesis is unknown. Here, we showed that depletion of the Rho signaling proteins MKLP1, MgcRacGAP, or ECT2 inhibited the localization of 1P-MRLC to the contractile ring but not the localization of 2P-MRLC to the midzone. In contrast, depleting or inhibiting a midzone-localizing kinase, Aurora B, perturbed the localization of 2P-MRLC to the midzone but not the localization of 1P-MRLC to the contractile ring. We did not observe any change in the localization of phosphorylated MRLC in myosin light-chain kinase (MLCK)-inhibited cells. Furrow regression was observed in Aurora B- and 2P-MRLC-inhibited cells but not in 1P-MRLC-perturbed dividing cells. Furthermore, Aurora B bound to 2P-MRLC in vitro and in vivo. These results suggest that Aurora B, but not Rho/MLCK signaling, is essential for the localization of 2P-MRLC to the midzone in dividing HeLa cells."xsd:string |
| http://purl.uniprot.org/citations/23951055 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0070965"xsd:string |
| http://purl.uniprot.org/citations/23951055 | http://purl.uniprot.org/core/author | "Kondo T."xsd:string |
| http://purl.uniprot.org/citations/23951055 | http://purl.uniprot.org/core/author | "Kamijo K."xsd:string |
| http://purl.uniprot.org/citations/23951055 | http://purl.uniprot.org/core/author | "Hosoya H."xsd:string |
| http://purl.uniprot.org/citations/23951055 | http://purl.uniprot.org/core/author | "Isoda R."xsd:string |
| http://purl.uniprot.org/citations/23951055 | http://purl.uniprot.org/core/author | "Hamao K."xsd:string |
| http://purl.uniprot.org/citations/23951055 | http://purl.uniprot.org/core/author | "Ookusa T."xsd:string |
| http://purl.uniprot.org/citations/23951055 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23951055 | http://purl.uniprot.org/core/name | "PLoS One"xsd:string |
| http://purl.uniprot.org/citations/23951055 | http://purl.uniprot.org/core/pages | "e70965"xsd:string |
| http://purl.uniprot.org/citations/23951055 | http://purl.uniprot.org/core/title | "Aurora B but not rho/MLCK signaling is required for localization of diphosphorylated myosin II regulatory light chain to the midzone in cytokinesis."xsd:string |
| http://purl.uniprot.org/citations/23951055 | http://purl.uniprot.org/core/volume | "8"xsd:string |
| http://purl.uniprot.org/citations/23951055 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23951055 |
| http://purl.uniprot.org/citations/23951055 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/23951055 |
| http://purl.uniprot.org/uniprot/#_A0A191TDH3-mappedCitation-23951055 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23951055 |
| http://purl.uniprot.org/uniprot/#_A0A7R6M6S5-mappedCitation-23951055 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23951055 |
| http://purl.uniprot.org/uniprot/#_A0A7R6M8Q8-mappedCitation-23951055 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23951055 |
| http://purl.uniprot.org/uniprot/#_A0A7R6RQK5-mappedCitation-23951055 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23951055 |
| http://purl.uniprot.org/uniprot/#_B2RC06-mappedCitation-23951055 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23951055 |
| http://purl.uniprot.org/uniprot/#_G3V1V8-mappedCitation-23951055 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23951055 |
| http://purl.uniprot.org/uniprot/#_D9ZGF8-mappedCitation-23951055 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23951055 |
| http://purl.uniprot.org/uniprot/#_Q14908-mappedCitation-23951055 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23951055 |
| http://purl.uniprot.org/uniprot/#_Q13464-mappedCitation-23951055 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23951055 |