| http://purl.uniprot.org/citations/23963154 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23963154 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23963154 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23963154 | http://www.w3.org/2000/01/rdf-schema#comment | "During the 10 days commencing April 29, 2013, the Iowa State University Veterinary Diagnostic Laboratory received the first 4 of many submissions from swine farms experiencing explosive epidemics of diarrhea and vomiting affecting all ages, with 90-95% mortality in suckling pigs. Histology revealed severe atrophy of villi in all segments of the small intestines with occasional villus-epithelial syncytial cells, but testing for rotaviruses and Transmissible gastroenteritis virus (Alphacoronavirus 1) were negative. Negative-staining electron microscopy of feces revealed coronavirus-like particles and a pan-coronavirus polymerase chain reaction (PCR) designed to amplify a conserved region of the polymerase gene for all members in the family Coronaviridae produced expected 251-bp amplicons. Subsequent sequencing and analysis revealed 99.6-100% identity among the PCR amplicons from the 4 farms and 97-99% identity to the corresponding portion of the polymerase gene of Porcine epidemic diarrhea virus (PEDV) strains, with the highest identity (99%) to strains from China in 2012. Findings were corroborated at National Veterinary Services Laboratories using 2 nested S-gene and 1 nested N-gene PCR tests where the sequenced amplicons also had the highest identity with 2012 China strains. Whole genome sequence for the virus from 2 farms in 2 different states using next-generation sequencing technique was compared to PEDV sequences available in GenBank. The 2013 U.S. PEDV had 96.6-99.5% identity with all known PEDV strains and the highest identity (>99.0%) to some of the 2011-2012 Chinese strains. The nearly simultaneous outbreaks of disease, and high degree of homology (99.6-100%) between the PEDV strains from the 4 unrelated farms, suggests a common source of virus."xsd:string |
| http://purl.uniprot.org/citations/23963154 | http://purl.org/dc/terms/identifier | "doi:10.1177/1040638713501675"xsd:string |
| http://purl.uniprot.org/citations/23963154 | http://purl.org/dc/terms/identifier | "doi:10.1177/1040638713501675"xsd:string |
| http://purl.uniprot.org/citations/23963154 | http://purl.org/dc/terms/identifier | "doi:10.1177/1040638713501675"xsd:string |
| http://purl.uniprot.org/citations/23963154 | http://purl.uniprot.org/core/author | "Sun D."xsd:string |
| http://purl.uniprot.org/citations/23963154 | http://purl.uniprot.org/core/author | "Sun D."xsd:string |
| http://purl.uniprot.org/citations/23963154 | http://purl.uniprot.org/core/author | "Sun D."xsd:string |
| http://purl.uniprot.org/citations/23963154 | http://purl.uniprot.org/core/author | "Schwartz K.J."xsd:string |
| http://purl.uniprot.org/citations/23963154 | http://purl.uniprot.org/core/author | "Schwartz K.J."xsd:string |
| http://purl.uniprot.org/citations/23963154 | http://purl.uniprot.org/core/author | "Schwartz K.J."xsd:string |
| http://purl.uniprot.org/citations/23963154 | http://purl.uniprot.org/core/author | "Yoon K.J."xsd:string |
| http://purl.uniprot.org/citations/23963154 | http://purl.uniprot.org/core/author | "Yoon K.J."xsd:string |
| http://purl.uniprot.org/citations/23963154 | http://purl.uniprot.org/core/author | "Yoon K.J."xsd:string |
| http://purl.uniprot.org/citations/23963154 | http://purl.uniprot.org/core/author | "Killian M.L."xsd:string |
| http://purl.uniprot.org/citations/23963154 | http://purl.uniprot.org/core/author | "Killian M.L."xsd:string |
| http://purl.uniprot.org/citations/23963154 | http://purl.uniprot.org/core/author | "Killian M.L."xsd:string |
| http://purl.uniprot.org/citations/23963154 | http://purl.uniprot.org/core/author | "Pillatzki A."xsd:string |
| http://purl.uniprot.org/citations/23963154 | http://purl.uniprot.org/core/author | "Pillatzki A."xsd:string |
| http://purl.uniprot.org/citations/23963154 | http://purl.uniprot.org/core/author | "Pillatzki A."xsd:string |
| http://purl.uniprot.org/citations/23963154 | http://purl.uniprot.org/core/author | "Burrough E.R."xsd:string |
| http://purl.uniprot.org/citations/23963154 | http://purl.uniprot.org/core/author | "Burrough E.R."xsd:string |
| http://purl.uniprot.org/citations/23963154 | http://purl.uniprot.org/core/author | "Burrough E.R."xsd:string |