| http://purl.uniprot.org/citations/23963449 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23963449 | http://www.w3.org/2000/01/rdf-schema#comment | "Versican G1 domain-containing fragments (VG1Fs) have been identified in extracts from the dermis in which hyaluronan (HA)-versican-fibrillin complexes are found. However, the molecular assembly of VG1Fs in the HA-versican-microfibril macrocomplex has not yet been elucidated. Here, we clarify the role of VG1Fs in the extracellular macrocomplex, specifically in mediating the recruitment of HA to microfibrils. Sequential extraction studies suggested that the VG1Fs were not associated with dermal elements through HA binding properties alone. Overlay analyses of dermal tissue sections using the recombinant versican G1 domain, rVN, showed that rVN deposited onto the elastic fiber network. In solid-phase binding assays, rVN bound to isolated nondegraded microfibrils. rVN specifically bound to authentic versican core protein produced by dermal fibroblasts. Furthermore, rVN bound to VG1Fs extracted from the dermis and to nondenatured versican but not to fibrillin-1. Homotypic binding of rVN was also seen. Consistent with these binding properties, macroaggregates containing VG1Fs were detected in high molecular weight fractions of sieved dermal extracts and visualized by electron microscopy, which revealed localization to microfibrils at the microscopic level. Importantly, exogenous rVN enhanced HA recruitment both to isolated microfibrils and to microfibrils in tissue sections in a dose-dependent manner. From these data, we propose that cleaved VG1Fs can be recaptured by microfibrils through VG1F homotypical interactions to enhance HA recruitment to microfibrils."xsd:string |
| http://purl.uniprot.org/citations/23963449 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m113.456947"xsd:string |
| http://purl.uniprot.org/citations/23963449 | http://purl.uniprot.org/core/author | "Watanabe K."xsd:string |
| http://purl.uniprot.org/citations/23963449 | http://purl.uniprot.org/core/author | "Yoneda M."xsd:string |
| http://purl.uniprot.org/citations/23963449 | http://purl.uniprot.org/core/author | "Kimata K."xsd:string |
| http://purl.uniprot.org/citations/23963449 | http://purl.uniprot.org/core/author | "Sakai L.Y."xsd:string |
| http://purl.uniprot.org/citations/23963449 | http://purl.uniprot.org/core/author | "Zako M."xsd:string |
| http://purl.uniprot.org/citations/23963449 | http://purl.uniprot.org/core/author | "Isogai Z."xsd:string |
| http://purl.uniprot.org/citations/23963449 | http://purl.uniprot.org/core/author | "Murasawa Y."xsd:string |
| http://purl.uniprot.org/citations/23963449 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23963449 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/23963449 | http://purl.uniprot.org/core/pages | "29170-29181"xsd:string |
| http://purl.uniprot.org/citations/23963449 | http://purl.uniprot.org/core/title | "Homotypic versican G1 domain interactions enhance hyaluronan incorporation into fibrillin microfibrils."xsd:string |
| http://purl.uniprot.org/citations/23963449 | http://purl.uniprot.org/core/volume | "288"xsd:string |
| http://purl.uniprot.org/citations/23963449 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23963449 |
| http://purl.uniprot.org/citations/23963449 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/23963449 |
| http://purl.uniprot.org/uniprot/#_A0A6M3WJI3-mappedCitation-23963449 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23963449 |
| http://purl.uniprot.org/uniprot/#_A0A1B1PFX2-mappedCitation-23963449 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23963449 |
| http://purl.uniprot.org/uniprot/#_A0A1B1PFX5-mappedCitation-23963449 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23963449 |
| http://purl.uniprot.org/uniprot/#_A0A384MDM4-mappedCitation-23963449 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23963449 |
| http://purl.uniprot.org/uniprot/#_B7Z3R6-mappedCitation-23963449 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23963449 |
| http://purl.uniprot.org/uniprot/#_B4E3I6-mappedCitation-23963449 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23963449 |
| http://purl.uniprot.org/uniprot/#_B7Z638-mappedCitation-23963449 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23963449 |
| http://purl.uniprot.org/uniprot/#_P13611-mappedCitation-23963449 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/23963449 |