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http://purl.uniprot.org/citations/2398054http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/2398054http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/2398054http://www.w3.org/2000/01/rdf-schema#comment"In the course of our studies on the structural diversity of the isoforms of cardiac fatty acid-binding proteins (cFABPs), a cardiac-type FABP from the matrix of bovine heart mitochondria was purified to homogeneity and obtained as a single 15-kDa protein with an isoelectric point of 4.9. The primary structures of this protein and of the two isoforms isolated from the cytosol (pI4.9-cFABP and pI 5.1-cFABP) were investigated by means of plasma desorption mass spectrometry and sequencing of peptides. All three proteins are amino-terminally blocked with an acetyl group and shown to be colinear with the sequence deduced from a cDNA clone for bovine heart fatty acid-binding protein (Billich, S., Wissel, T., Kratzin, H., Hahn, U., Hagenhoff, B., Lezius, A. G., and Spener, F. (1988) Eur. J. Biochem. 175, 549-556) except for the residue at position 98. This residue is demonstrated to be the molecular origin of bovine cFABP isoforms since pI 5.1-cFABP contains Asn98 in accordance with the sequence derived from the cDNA, whereas in pI 4.9-cFABP, this position is occupied by Asp98. Moreover, mitochondrial FABP is identical to pI 4.9-cFABP. Molecular masses of pI 4.9-cFABP (14,679 +/- 10 Da) and pI 5.1-cFABP (14,678 +/-20 Da) determined by plasma desorption mass spectrometry coincide with that calculated from the cDNA (14,673 Da). Hence, residues linked to these proteins by posttranslational modification are not present, and the Asn-Asp exchange is the sole origin of heterogeneity of mitochondrial and cytosolic fatty acid-binding proteins from bovine heart."xsd:string
http://purl.uniprot.org/citations/2398054http://purl.org/dc/terms/identifier"doi:10.1016/s0021-9258(17)46216-8"xsd:string
http://purl.uniprot.org/citations/2398054http://purl.org/dc/terms/identifier"doi:10.1016/s0021-9258(17)46216-8"xsd:string
http://purl.uniprot.org/citations/2398054http://purl.uniprot.org/core/author"Roepstorff P."xsd:string
http://purl.uniprot.org/citations/2398054http://purl.uniprot.org/core/author"Roepstorff P."xsd:string
http://purl.uniprot.org/citations/2398054http://purl.uniprot.org/core/author"Hoejrup P."xsd:string
http://purl.uniprot.org/citations/2398054http://purl.uniprot.org/core/author"Hoejrup P."xsd:string
http://purl.uniprot.org/citations/2398054http://purl.uniprot.org/core/author"Knudsen J."xsd:string
http://purl.uniprot.org/citations/2398054http://purl.uniprot.org/core/author"Knudsen J."xsd:string
http://purl.uniprot.org/citations/2398054http://purl.uniprot.org/core/author"Spener F."xsd:string
http://purl.uniprot.org/citations/2398054http://purl.uniprot.org/core/author"Spener F."xsd:string
http://purl.uniprot.org/citations/2398054http://purl.uniprot.org/core/author"Boerchers T."xsd:string
http://purl.uniprot.org/citations/2398054http://purl.uniprot.org/core/author"Boerchers T."xsd:string
http://purl.uniprot.org/citations/2398054http://purl.uniprot.org/core/author"Unterberg C."xsd:string
http://purl.uniprot.org/citations/2398054http://purl.uniprot.org/core/author"Unterberg C."xsd:string
http://purl.uniprot.org/citations/2398054http://purl.uniprot.org/core/date"1990"xsd:gYear
http://purl.uniprot.org/citations/2398054http://purl.uniprot.org/core/date"1990"xsd:gYear
http://purl.uniprot.org/citations/2398054http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
http://purl.uniprot.org/citations/2398054http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
http://purl.uniprot.org/citations/2398054http://purl.uniprot.org/core/pages"16255-16261"xsd:string
http://purl.uniprot.org/citations/2398054http://purl.uniprot.org/core/pages"16255-16261"xsd:string
http://purl.uniprot.org/citations/2398054http://purl.uniprot.org/core/title"Cardiac fatty acid-binding proteins. Isolation and characterization of the mitochondrial fatty acid-binding protein and its structural relationship with the cytosolic isoforms."xsd:string
http://purl.uniprot.org/citations/2398054http://purl.uniprot.org/core/title"Cardiac fatty acid-binding proteins. Isolation and characterization of the mitochondrial fatty acid-binding protein and its structural relationship with the cytosolic isoforms."xsd:string
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