| http://purl.uniprot.org/citations/23986595 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/23986595 | http://www.w3.org/2000/01/rdf-schema#comment | "Hepatitis C virus (HCV) orchestrates the different stages of its life cycle in time and space through the sequential participation of HCV proteins and cellular machineries; hence, these represent tractable molecular host targets for HCV elimination by combination therapies. We recently identified multifunctional Y-box-binding protein 1 (YB-1 or YBX1) as an interacting partner of NS3/4A protein and HCV genomic RNA that negatively regulates the equilibrium between viral translation/replication and particle production. To identify novel host factors that regulate the production of infectious particles, we elucidated the YB-1 interactome in human hepatoma cells by a quantitative mass spectrometry approach. We identified 71 YB-1-associated proteins that included previously reported HCV regulators DDX3, heterogeneous nuclear RNP A1, and ILF2. Of the potential YB-1 interactors, 26 proteins significantly modulated HCV replication in a gene-silencing screening. Following extensive interaction and functional validation, we identified three YB-1 partners, C1QBP, LARP-1, and IGF2BP2, that redistribute to the surface of core-containing lipid droplets in HCV JFH-1-expressing cells, similarly to YB-1 and DDX6. Importantly, knockdown of these proteins stimulated the release and/or egress of HCV particles without affecting virus assembly, suggesting a functional YB-1 protein complex that negatively regulates virus production. Furthermore, a JFH-1 strain with the NS3 Q221L mutation, which promotes virus production, was less sensitive to this negative regulation, suggesting that this HCV-specific YB-1 protein complex modulates an NS3-dependent step in virus production. Overall, our data support a model in which HCV hijacks host cell machinery containing numerous RNA-binding proteins to control the equilibrium between viral RNA replication and NS3-dependent late steps in particle production."xsd:string |
| http://purl.uniprot.org/citations/23986595 | http://purl.org/dc/terms/identifier | "doi:10.1128/jvi.01474-13"xsd:string |
| http://purl.uniprot.org/citations/23986595 | http://purl.uniprot.org/core/author | "Thibault P."xsd:string |
| http://purl.uniprot.org/citations/23986595 | http://purl.uniprot.org/core/author | "Chatel-Chaix L."xsd:string |
| http://purl.uniprot.org/citations/23986595 | http://purl.uniprot.org/core/author | "Lamarre D."xsd:string |
| http://purl.uniprot.org/citations/23986595 | http://purl.uniprot.org/core/author | "Bonneil E."xsd:string |
| http://purl.uniprot.org/citations/23986595 | http://purl.uniprot.org/core/author | "Baril M."xsd:string |
| http://purl.uniprot.org/citations/23986595 | http://purl.uniprot.org/core/author | "Motorina A."xsd:string |
| http://purl.uniprot.org/citations/23986595 | http://purl.uniprot.org/core/author | "Germain M.A."xsd:string |
| http://purl.uniprot.org/citations/23986595 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/23986595 | http://purl.uniprot.org/core/name | "J Virol"xsd:string |
| http://purl.uniprot.org/citations/23986595 | http://purl.uniprot.org/core/pages | "11704-11720"xsd:string |
| http://purl.uniprot.org/citations/23986595 | http://purl.uniprot.org/core/title | "A host YB-1 ribonucleoprotein complex is hijacked by hepatitis C virus for the control of NS3-dependent particle production."xsd:string |
| http://purl.uniprot.org/citations/23986595 | http://purl.uniprot.org/core/volume | "87"xsd:string |
| http://purl.uniprot.org/citations/23986595 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/23986595 |
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