| http://purl.uniprot.org/citations/24013648 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24013648 | http://www.w3.org/2000/01/rdf-schema#comment | "Caveolae are an abundant feature of the plasma membrane of many mammalian cell types, and have key roles in mechano-transduction, metabolic regulation, and vascular permeability. Caveolin and cavin proteins, as well as EHD2 and pacsin 2, are all present in caveolae. How these proteins assemble to form a protein interaction network for caveolar morphogenesis is not known. Using in vivo crosslinking, velocity gradient centrifugation, immuno-isolation, and tandem mass spectrometry, we determine that cavins and caveolins assemble into a homogenous 80S complex, which we term the caveolar coat complex. There are no further abundant components within this complex, and the complex excludes EHD2 and pacsin 2. Cavin 1 forms trimers and interacts with caveolin 1 with a molar ratio of about 1∶4. Cavins 2 and 3 compete for binding sites within the overall coat complex, and form distinct subcomplexes with cavin 1. The core interactions between caveolin 1 and cavin 1 are independent of cavin 2, cavin 3, and EHD2 expression, and the cavins themselves can still interact in the absence of caveolin 1. Using immuno-electron microscopy as well as a recently developed protein tag for electron microscopy (MiniSOG), we demonstrate that caveolar coat complexes form a distinct coat all around the caveolar bulb. In contrast, and consistent with our biochemical data, EHD2 defines a different domain at the caveolar neck. 3D electron tomograms of the caveolar coat, labeled using cavin-MiniSOG, show that the caveolar coat is composed of repeating units of a unitary caveolar coat complex."xsd:string |
| http://purl.uniprot.org/citations/24013648 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pbio.1001640"xsd:string |
| http://purl.uniprot.org/citations/24013648 | http://purl.uniprot.org/core/author | "Ellisman M.H."xsd:string |
| http://purl.uniprot.org/citations/24013648 | http://purl.uniprot.org/core/author | "Mackey M."xsd:string |
| http://purl.uniprot.org/citations/24013648 | http://purl.uniprot.org/core/author | "Ludwig A."xsd:string |
| http://purl.uniprot.org/citations/24013648 | http://purl.uniprot.org/core/author | "Nichols B.J."xsd:string |
| http://purl.uniprot.org/citations/24013648 | http://purl.uniprot.org/core/author | "Howard G."xsd:string |
| http://purl.uniprot.org/citations/24013648 | http://purl.uniprot.org/core/author | "Deerinck T."xsd:string |
| http://purl.uniprot.org/citations/24013648 | http://purl.uniprot.org/core/author | "Sandin S."xsd:string |
| http://purl.uniprot.org/citations/24013648 | http://purl.uniprot.org/core/author | "Mendoza-Topaz C."xsd:string |
| http://purl.uniprot.org/citations/24013648 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/24013648 | http://purl.uniprot.org/core/name | "PLoS Biol"xsd:string |
| http://purl.uniprot.org/citations/24013648 | http://purl.uniprot.org/core/pages | "e1001640"xsd:string |
| http://purl.uniprot.org/citations/24013648 | http://purl.uniprot.org/core/title | "Molecular composition and ultrastructure of the caveolar coat complex."xsd:string |
| http://purl.uniprot.org/citations/24013648 | http://purl.uniprot.org/core/volume | "11"xsd:string |
| http://purl.uniprot.org/citations/24013648 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/24013648 |
| http://purl.uniprot.org/citations/24013648 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/24013648 |
| http://purl.uniprot.org/uniprot/P51636#attribution-F63BDA5BD1A71211661313C8F4D3C09D | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/24013648 |
| http://purl.uniprot.org/uniprot/Q91VJ2#attribution-D7F383BB1D6303E34BC036A8C7345F5A | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/24013648 |
| http://purl.uniprot.org/uniprot/O54724#attribution-D7F383BB1D6303E34BC036A8C7345F5A | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/24013648 |
| http://purl.uniprot.org/uniprot/Q6NZI2#attribution-5DD82E7E471C53C8F35674961AD639E9 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/24013648 |
| http://purl.uniprot.org/uniprot/Q6NZI2#attribution-F63BDA5BD1A71211661313C8F4D3C09D | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/24013648 |
| http://purl.uniprot.org/uniprot/Q969G5#attribution-F63BDA5BD1A71211661313C8F4D3C09D | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/24013648 |
| http://purl.uniprot.org/uniprot/Q14254#attribution-F63BDA5BD1A71211661313C8F4D3C09D | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/24013648 |