http://purl.uniprot.org/citations/24038867 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/24038867 | http://www.w3.org/2000/01/rdf-schema#comment | "BackgroundIncreased insecticide detoxification mediated by cytochrome P450s is a common mechanism of insecticide resistance. Although Cyp6a2 has been observed to be overexpressed in many 4,4'-dichlorodiphenyltrichloroethane (DDT)-resistant strains of Drosophila melanogaster, how Cyp6a2 is regulated and whether its overproduction confers DDT resistance remain elusive.ResultsMolecular analysis identified five Cyp6a2 alleles (Cyp6a2(Canton) (-S-1) , Cyp6a2(Canton) (-S-2) , Cyp6a2(91-C) , Cyp6a2(91-R) and Cyp6a2(Wisconsin) (-) (WD) ) from four D. melanogaster strains, notably differing in the presence or absence of an intact Nrf2/Maf (a transcription factor) binding site in the 5'-promoter core region, a 'G1410' frameshift deletion mutation in the heme-binding region and a long terminal repeat (LTR) of transposable element 17.6 in the 3'-untranslated region (UTR). Linkage analysis confirmed that DDT resistance was genetically linked to a Nrf2/Maf-binding-site-containing, LTR-lacking functional allele of Cyp6a2 (Cyp6a2(91-R) ). The qRT-PCR results showed that overexpression of functional Cyp6a2 was consistently associated with DDT resistance. Luciferase reporter gene assays revealed that an intact Nrf2/Maf binding site in the 5'-promoter core region enhanced the constitutive transcription of Cyp6a2.ConclusionThe results suggest that the Nrf2/Maf binding-site-containing functional Cyp6a2 allele is associated with DDT resistance in the D. melanogaster strains under study."xsd:string |
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http://purl.uniprot.org/citations/24038867 | http://purl.uniprot.org/core/author | "Liu Y."xsd:string |
http://purl.uniprot.org/citations/24038867 | http://purl.uniprot.org/core/author | "Li M."xsd:string |
http://purl.uniprot.org/citations/24038867 | http://purl.uniprot.org/core/author | "Li X."xsd:string |
http://purl.uniprot.org/citations/24038867 | http://purl.uniprot.org/core/author | "Zhu S."xsd:string |
http://purl.uniprot.org/citations/24038867 | http://purl.uniprot.org/core/author | "Qiu X."xsd:string |
http://purl.uniprot.org/citations/24038867 | http://purl.uniprot.org/core/author | "Wan H."xsd:string |
http://purl.uniprot.org/citations/24038867 | http://purl.uniprot.org/core/author | "Pittendrigh B.R."xsd:string |
http://purl.uniprot.org/citations/24038867 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
http://purl.uniprot.org/citations/24038867 | http://purl.uniprot.org/core/name | "Pest Manag Sci"xsd:string |
http://purl.uniprot.org/citations/24038867 | http://purl.uniprot.org/core/pages | "1048-1058"xsd:string |
http://purl.uniprot.org/citations/24038867 | http://purl.uniprot.org/core/title | "Nrf2/Maf-binding-site-containing functional Cyp6a2 allele is associated with DDT resistance in Drosophila melanogaster."xsd:string |
http://purl.uniprot.org/citations/24038867 | http://purl.uniprot.org/core/volume | "70"xsd:string |
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