| http://purl.uniprot.org/citations/2406247 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2406247 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2406247 | http://www.w3.org/2000/01/rdf-schema#comment | "Replication protein A (RP-A) is a complex of three polypeptides of molecular mass 70, 32, and 14 kDa, which is absolutely required for simian virus 40 DNA replication in vitro. We have isolated a cDNA coding for the 32-kDa subunit of RP-A. An oligonucleotide probe was constructed based upon a tryptic peptide sequence derived from whole RP-A, and clones were isolated from a lambda gt11 library containing HeLa cDNA inserts. The amino acid sequence predicted from the cDNA contains the peptide sequence obtained from whole RP-A along with two sequences obtained from tryptic peptides derived from sodium dodecyl sulfate-polyacrylamide gel-purified 32-kDa subunit. The coding sequence predicts a protein of 29,228 daltons, in good agreement with the electrophoretically determined molecular mass of the 32-kDa subunit. No significant homology was found with any of the sequences in the GenBank data base. The protein predicted from the cDNA has an N-terminal region rich in glycine and serine along with two acidic and two basic segments. Monoclonal antibodies have been raised against the 70- and 32-kDa subunits of RP-A. The cloned cDNA has been overexpressed in bacteria using an inducible T7 expression system. The protein made in bacteria is recognized by a monoclonal antibody that is specific for the 32-kDa subunit of RP-A. This monoclonal antibody against the 32-kDa subunit inhibits DNA replication in vitro."xsd:string |
| http://purl.uniprot.org/citations/2406247 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(19)39750-9"xsd:string |
| http://purl.uniprot.org/citations/2406247 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(19)39750-9"xsd:string |
| http://purl.uniprot.org/citations/2406247 | http://purl.uniprot.org/core/author | "Kelly T.J."xsd:string |
| http://purl.uniprot.org/citations/2406247 | http://purl.uniprot.org/core/author | "Kelly T.J."xsd:string |
| http://purl.uniprot.org/citations/2406247 | http://purl.uniprot.org/core/author | "Wold M.S."xsd:string |
| http://purl.uniprot.org/citations/2406247 | http://purl.uniprot.org/core/author | "Wold M.S."xsd:string |
| http://purl.uniprot.org/citations/2406247 | http://purl.uniprot.org/core/author | "Erdile L.F."xsd:string |
| http://purl.uniprot.org/citations/2406247 | http://purl.uniprot.org/core/author | "Erdile L.F."xsd:string |
| http://purl.uniprot.org/citations/2406247 | http://purl.uniprot.org/core/date | "1990"xsd:gYear |
| http://purl.uniprot.org/citations/2406247 | http://purl.uniprot.org/core/date | "1990"xsd:gYear |
| http://purl.uniprot.org/citations/2406247 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/2406247 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/2406247 | http://purl.uniprot.org/core/pages | "3177-3182"xsd:string |
| http://purl.uniprot.org/citations/2406247 | http://purl.uniprot.org/core/pages | "3177-3182"xsd:string |
| http://purl.uniprot.org/citations/2406247 | http://purl.uniprot.org/core/title | "The primary structure of the 32-kDa subunit of human replication protein A."xsd:string |
| http://purl.uniprot.org/citations/2406247 | http://purl.uniprot.org/core/title | "The primary structure of the 32-kDa subunit of human replication protein A."xsd:string |
| http://purl.uniprot.org/citations/2406247 | http://purl.uniprot.org/core/volume | "265"xsd:string |
| http://purl.uniprot.org/citations/2406247 | http://purl.uniprot.org/core/volume | "265"xsd:string |
| http://purl.uniprot.org/citations/2406247 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/2406247 |
| http://purl.uniprot.org/citations/2406247 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/2406247 |
| http://purl.uniprot.org/citations/2406247 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/2406247 |
| http://purl.uniprot.org/citations/2406247 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/2406247 |