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http://purl.uniprot.org/citations/24089996http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/24089996http://www.w3.org/2000/01/rdf-schema#comment"

Background

Mycobacterium tuberculosis (Mtb) infections are still a major cause of death among all infectious diseases. Although 99% of individuals infected with Mtb develop a CD4(+) Th1 and CD8(+) T cell mediated immunity as measured by tuberculin skin test, this results only in partial protection and Mtb vaccines are not effective. Deviation of immune responses by pathogens towards a Th2 profile is a common mechanism of immune evasion, typically leading to the persistence of the microbes.

Results

Here we tested the stimulatory capacity of selective Mtb antigens on human monocyte-derived dendritic cell (DC) maturation and cytokine production. DC maturation markers CD80, CD86 and CD83 were readily upregulated by H37Ra- and H37Rv-associated antigens, the 30-kDa (from Ag85 B complex) and 38-KDa Mtb antigens only partially induced these markers. All Mtb antigens induced variable levels of IL-6 and low levels of IL-10, there was no release of IL-12p70 detectable. Substantial IL-12p40 production was restricted to LPS or H37Ra and H37Rv preparations. Although the proliferation levels of primary T cell responses were comparable using all the differentially stimulated DC, the 30-kDa and 38-kDa antigens showed a bias towards IL-4 secretion of polarized CD4(+) T cells after secondary stimulation as compared to H37Ra and H37Rv preparations.

Conclusion

Together our data indicate that 30-kDa and 38-kDa Mtb antigens induced only partial DC maturation shifting immune responses towards a Th2 profile."xsd:string
http://purl.uniprot.org/citations/24089996http://purl.org/dc/terms/identifier"doi:10.1186/1471-2172-14-48"xsd:string
http://purl.uniprot.org/citations/24089996http://purl.uniprot.org/core/author"Lee J.S."xsd:string
http://purl.uniprot.org/citations/24089996http://purl.uniprot.org/core/author"Jo E.K."xsd:string
http://purl.uniprot.org/citations/24089996http://purl.uniprot.org/core/author"Lutz M.B."xsd:string
http://purl.uniprot.org/citations/24089996http://purl.uniprot.org/core/author"Heuer M."xsd:string
http://purl.uniprot.org/citations/24089996http://purl.uniprot.org/core/author"Ribechini E."xsd:string
http://purl.uniprot.org/citations/24089996http://purl.uniprot.org/core/author"Behlich A.S."xsd:string
http://purl.uniprot.org/citations/24089996http://purl.uniprot.org/core/date"2013"xsd:gYear
http://purl.uniprot.org/citations/24089996http://purl.uniprot.org/core/name"BMC Immunol"xsd:string
http://purl.uniprot.org/citations/24089996http://purl.uniprot.org/core/pages"48"xsd:string
http://purl.uniprot.org/citations/24089996http://purl.uniprot.org/core/title"The 30-kDa and 38-kDa antigens from Mycobacterium tuberculosis induce partial maturation of human dendritic cells shifting CD4(+) T cell responses towards IL-4 production."xsd:string
http://purl.uniprot.org/citations/24089996http://purl.uniprot.org/core/volume"14"xsd:string
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