| http://purl.uniprot.org/citations/24097825 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24097825 | http://www.w3.org/2000/01/rdf-schema#comment | "Bcl-2 is phosphorylated on Ser(70) after treatment of cells with spindle poisons. On the basis of effects observed in cells overexpressing Bcl-2 S70E or S70A mutants, various studies have concluded that Ser(70) phosphorylation either enhances or diminishes Bcl-2 function. In the present study, the ability of phosphorylated Bcl-2, as well as the S70E and S70A mutants, to bind and neutralize proapoptotic Bcl-2 family members under cell-free conditions and in intact cells was examined in an attempt to resolve this controversy. Surface plasmon resonance indicated that phosphorylated Bcl-2, Bcl-2 S70E, and Bcl-2 S70A exhibit enhanced binding to Bim and Bak compared with unmodified Bcl-2. This enhanced binding reflected a readily detectable conformation change in the loop domain of Bcl-2. Furthermore, Bcl-2 S70E and S70A bound more Bak and Bim than wild-type Bcl-2 in pull-downs and afforded greater protection against several chemotherapeutic agents. Importantly, binding of endogenous Bcl-2 to Bim also increased during mitosis, when Bcl-2 is endogenously phosphorylated, and disruption of this mitotic Bcl-2/Bim binding with navitoclax or ABT-199, like Bcl-2 downregulation, enhanced the cytotoxicity of paclitaxel. Collectively, these results provide not only a mechanistic basis for the enhanced antiapoptotic activity of phosphorylated Bcl-2, but also an explanation for the ability of BH3 mimetics to enhance taxane sensitivity."xsd:string |
| http://purl.uniprot.org/citations/24097825 | http://purl.org/dc/terms/identifier | "doi:10.1158/0008-5472.can-13-0940"xsd:string |
| http://purl.uniprot.org/citations/24097825 | http://purl.uniprot.org/core/author | "Lee S.H."xsd:string |
| http://purl.uniprot.org/citations/24097825 | http://purl.uniprot.org/core/author | "Kaufmann S.H."xsd:string |
| http://purl.uniprot.org/citations/24097825 | http://purl.uniprot.org/core/author | "Ding H."xsd:string |
| http://purl.uniprot.org/citations/24097825 | http://purl.uniprot.org/core/author | "Dai H."xsd:string |
| http://purl.uniprot.org/citations/24097825 | http://purl.uniprot.org/core/author | "Meng X.W."xsd:string |
| http://purl.uniprot.org/citations/24097825 | http://purl.uniprot.org/core/author | "Schneider P.A."xsd:string |
| http://purl.uniprot.org/citations/24097825 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/24097825 | http://purl.uniprot.org/core/name | "Cancer Res"xsd:string |
| http://purl.uniprot.org/citations/24097825 | http://purl.uniprot.org/core/pages | "6998-7008"xsd:string |
| http://purl.uniprot.org/citations/24097825 | http://purl.uniprot.org/core/title | "Contribution of Bcl-2 phosphorylation to Bak binding and drug resistance."xsd:string |
| http://purl.uniprot.org/citations/24097825 | http://purl.uniprot.org/core/volume | "73"xsd:string |
| http://purl.uniprot.org/citations/24097825 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/24097825 |
| http://purl.uniprot.org/citations/24097825 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/24097825 |
| http://purl.uniprot.org/uniprot/#_A0A1L4AQQ4-mappedCitation-24097825 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24097825 |
| http://purl.uniprot.org/uniprot/#_A0A0S2Z319-mappedCitation-24097825 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24097825 |
| http://purl.uniprot.org/uniprot/#_P10415-mappedCitation-24097825 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24097825 |
| http://purl.uniprot.org/uniprot/#_A0A0S2Z391-mappedCitation-24097825 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24097825 |
| http://purl.uniprot.org/uniprot/#_A0A1L4AQQ5-mappedCitation-24097825 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24097825 |
| http://purl.uniprot.org/uniprot/#_A0A1L4AQQ8-mappedCitation-24097825 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24097825 |
| http://purl.uniprot.org/uniprot/#_A0A1L4AQR6-mappedCitation-24097825 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24097825 |
| http://purl.uniprot.org/uniprot/#_A0A7I2V3S7-mappedCitation-24097825 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24097825 |
| http://purl.uniprot.org/uniprot/#_A9QXG9-mappedCitation-24097825 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24097825 |