| http://purl.uniprot.org/citations/24098488 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24098488 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24098488 | http://www.w3.org/2000/01/rdf-schema#comment | "O-linked β-N-acetylglucosamine (O-GlcNAc) is an important post-translational modification (PTM) consisting of a single N-acetylglucosamine moiety attached via an O-β-glycosidic linkage to serine and threonine residues. Glycosylation with O-GlcNAc occurs on myriad nuclear and cytosolic proteins from almost all functional classes. However, with respect to O-GlcNAcylated proteins special in mitochondria, little attention has been paid. In this study, we combined mass spectrometry and immunological methods to perform global exploration of O-GlcNAcylated proteins specific in mitochondria of rat liver. First, highly purified mitochondrial proteins were obviously shown to be O-GlcNAcylated by immunoblot profiling. Then, β-elimination followed by Michael Addition with Dithiothreitol (BEMAD) treatment and LC-MS/MS were performed to enrich and identify O-GlcNAcylated mitochondrial proteins, resulting in an unambiguous assignment of 14 O-GlcNAcylation sites, mapping to 11 O-GlcNAcylated proteins. Furthermore, the identified O-GlcNAcylated mitochondrial proteins were fully validated by both electron transfer dissociation tandem mass spectrometry (ETD/MS/MS) and western blot. Thus, for the first time, our study definitely not only identified but also validated that some mitochondrial proteins in rat liver are O-GlcNAcylated. Interestingly, all of these O-GlcNAcylated mitochondrial proteins are enzymes, the majority of which are involved in a wide variety of biological processes, such as urea cycle, tricarboxylic acid cycle and lipid metabolism, indicating a role for protein O-GlcNAcylation in mitochondrial function."xsd:string |
| http://purl.uniprot.org/citations/24098488 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0076399"xsd:string |
| http://purl.uniprot.org/citations/24098488 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0076399"xsd:string |
| http://purl.uniprot.org/citations/24098488 | http://purl.uniprot.org/core/author | "Cao J."xsd:string |
| http://purl.uniprot.org/citations/24098488 | http://purl.uniprot.org/core/author | "Cao J."xsd:string |
| http://purl.uniprot.org/citations/24098488 | http://purl.uniprot.org/core/author | "Huang J."xsd:string |
| http://purl.uniprot.org/citations/24098488 | http://purl.uniprot.org/core/author | "Huang J."xsd:string |
| http://purl.uniprot.org/citations/24098488 | http://purl.uniprot.org/core/author | "Xu H."xsd:string |
| http://purl.uniprot.org/citations/24098488 | http://purl.uniprot.org/core/author | "Xu H."xsd:string |
| http://purl.uniprot.org/citations/24098488 | http://purl.uniprot.org/core/author | "Yan G."xsd:string |
| http://purl.uniprot.org/citations/24098488 | http://purl.uniprot.org/core/author | "Yan G."xsd:string |
| http://purl.uniprot.org/citations/24098488 | http://purl.uniprot.org/core/author | "Yang P."xsd:string |
| http://purl.uniprot.org/citations/24098488 | http://purl.uniprot.org/core/author | "Yang P."xsd:string |
| http://purl.uniprot.org/citations/24098488 | http://purl.uniprot.org/core/author | "Cao W."xsd:string |
| http://purl.uniprot.org/citations/24098488 | http://purl.uniprot.org/core/author | "Cao W."xsd:string |
| http://purl.uniprot.org/citations/24098488 | http://purl.uniprot.org/core/author | "Yao J."xsd:string |
| http://purl.uniprot.org/citations/24098488 | http://purl.uniprot.org/core/author | "Yao J."xsd:string |
| http://purl.uniprot.org/citations/24098488 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/24098488 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/24098488 | http://purl.uniprot.org/core/name | "PLoS ONE"xsd:string |
| http://purl.uniprot.org/citations/24098488 | http://purl.uniprot.org/core/name | "PLoS ONE"xsd:string |
| http://purl.uniprot.org/citations/24098488 | http://purl.uniprot.org/core/pages | "E76399"xsd:string |
| http://purl.uniprot.org/citations/24098488 | http://purl.uniprot.org/core/pages | "E76399"xsd:string |