| http://purl.uniprot.org/citations/24100027 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24100027 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24100027 | http://www.w3.org/2000/01/rdf-schema#comment | "Type III polyketide synthases (PKSs) show diverse cyclization specificity. We previously characterized two Azotobacter type III PKSs (ArsB and ArsC) with different cyclization specificity. ArsB and ArsC, which share a high sequence identity (71%), produce alkylresorcinols and alkylpyrones through aldol condensation and lactonization of the same polyketomethylene intermediate, respectively. Here we identified a key amino acid residue for the cyclization specificity of each enzyme by site-directed mutagenesis. Trp-281 of ArsB corresponded to Gly-284 of ArsC in the amino acid sequence alignment. The ArsB W281G mutant synthesized alkylpyrone but not alkylresorcinol. In contrast, the ArsC G284W mutant synthesized alkylresorcinol with a small amount of alkylpyrone. These results indicate that this amino acid residue (Trp-281 of ArsB or Gly-284 of ArsC) should occupy a critical position for the cyclization specificity of each enzyme. We then determined crystal structures of the wild-type and G284W ArsC proteins at resolutions of 1.76 and 1.99 Å, respectively. Comparison of these two ArsC structures indicates that the G284W substitution brings a steric wall to the active site cavity, resulting in a significant reduction of the cavity volume. We postulate that the polyketomethylene intermediate can be folded to a suitable form for aldol condensation only in such a relatively narrow cavity of ArsC G284W (and presumably ArsB). This is the first report on the alteration of cyclization specificity from lactonization to aldol condensation for a type III PKS. The ArsC G284W structure is significant as it is the first reported structure of a microbial resorcinol synthase."xsd:string |
| http://purl.uniprot.org/citations/24100027 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.M113.487272"xsd:string |
| http://purl.uniprot.org/citations/24100027 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m113.487272"xsd:string |
| http://purl.uniprot.org/citations/24100027 | http://purl.uniprot.org/core/author | "Horinouchi S."xsd:string |
| http://purl.uniprot.org/citations/24100027 | http://purl.uniprot.org/core/author | "Horinouchi S."xsd:string |
| http://purl.uniprot.org/citations/24100027 | http://purl.uniprot.org/core/author | "Ohnishi Y."xsd:string |
| http://purl.uniprot.org/citations/24100027 | http://purl.uniprot.org/core/author | "Ohnishi Y."xsd:string |
| http://purl.uniprot.org/citations/24100027 | http://purl.uniprot.org/core/author | "Katsuyama Y."xsd:string |
| http://purl.uniprot.org/citations/24100027 | http://purl.uniprot.org/core/author | "Katsuyama Y."xsd:string |
| http://purl.uniprot.org/citations/24100027 | http://purl.uniprot.org/core/author | "Miyazono K.I."xsd:string |
| http://purl.uniprot.org/citations/24100027 | http://purl.uniprot.org/core/author | "Tanokura M."xsd:string |
| http://purl.uniprot.org/citations/24100027 | http://purl.uniprot.org/core/author | "Tanokura M."xsd:string |
| http://purl.uniprot.org/citations/24100027 | http://purl.uniprot.org/core/author | "Funa N."xsd:string |
| http://purl.uniprot.org/citations/24100027 | http://purl.uniprot.org/core/author | "Funa N."xsd:string |
| http://purl.uniprot.org/citations/24100027 | http://purl.uniprot.org/core/author | "Miyanaga A."xsd:string |
| http://purl.uniprot.org/citations/24100027 | http://purl.uniprot.org/core/author | "Miyanaga A."xsd:string |
| http://purl.uniprot.org/citations/24100027 | http://purl.uniprot.org/core/author | "Miyazono K."xsd:string |
| http://purl.uniprot.org/citations/24100027 | http://purl.uniprot.org/core/author | "Ozawa H."xsd:string |
| http://purl.uniprot.org/citations/24100027 | http://purl.uniprot.org/core/author | "Ozawa H."xsd:string |
| http://purl.uniprot.org/citations/24100027 | http://purl.uniprot.org/core/author | "Satou R."xsd:string |
| http://purl.uniprot.org/citations/24100027 | http://purl.uniprot.org/core/author | "Satou R."xsd:string |
| http://purl.uniprot.org/citations/24100027 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/24100027 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |