| http://purl.uniprot.org/citations/24106281 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24106281 | http://www.w3.org/2000/01/rdf-schema#comment | "Mammalian genomes encode two provitamin A-converting enzymes as follows: the β-carotene-15,15'-oxygenase (BCO1) and the β-carotene-9',10'-oxygenase (BCO2). Symmetric cleavage by BCO1 yields retinoids (β-15'-apocarotenoids, C20), whereas eccentric cleavage by BCO2 produces long-chain (>C20) apocarotenoids. Here, we used genetic and biochemical approaches to clarify the contribution of these enzymes to provitamin A metabolism. We subjected wild type, Bco1(-/-), Bco2(-/-), and Bco1(-/-)Bco2(-/-) double knock-out mice to a controlled diet providing β-carotene as the sole source for apocarotenoid production. This study revealed that BCO1 is critical for retinoid homeostasis. Genetic disruption of BCO1 resulted in β-carotene accumulation and vitamin A deficiency accompanied by a BCO2-dependent production of minor amounts of β-apo-10'-carotenol (APO10ol). We found that APO10ol can be esterified and transported by the same proteins as vitamin A but with a lower affinity and slower reaction kinetics. In wild type mice, APO10ol was converted to retinoids by BCO1. We also show that a stepwise cleavage by BCO2 and BCO1 with APO10ol as an intermediate could provide a mechanism to tailor asymmetric carotenoids such as β-cryptoxanthin for vitamin A production. In conclusion, our study provides evidence that mammals employ both carotenoid oxygenases to synthesize retinoids from provitamin A carotenoids."xsd:string |
| http://purl.uniprot.org/citations/24106281 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m113.501049"xsd:string |
| http://purl.uniprot.org/citations/24106281 | http://purl.uniprot.org/core/author | "Palczewski K."xsd:string |
| http://purl.uniprot.org/citations/24106281 | http://purl.uniprot.org/core/author | "von Lintig J."xsd:string |
| http://purl.uniprot.org/citations/24106281 | http://purl.uniprot.org/core/author | "Golczak M."xsd:string |
| http://purl.uniprot.org/citations/24106281 | http://purl.uniprot.org/core/author | "Amengual J."xsd:string |
| http://purl.uniprot.org/citations/24106281 | http://purl.uniprot.org/core/author | "Hessel S."xsd:string |
| http://purl.uniprot.org/citations/24106281 | http://purl.uniprot.org/core/author | "Widjaja-Adhi M.A.K."xsd:string |
| http://purl.uniprot.org/citations/24106281 | http://purl.uniprot.org/core/author | "Rodriguez-Santiago S."xsd:string |
| http://purl.uniprot.org/citations/24106281 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/24106281 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/24106281 | http://purl.uniprot.org/core/pages | "34081-34096"xsd:string |
| http://purl.uniprot.org/citations/24106281 | http://purl.uniprot.org/core/title | "Two carotenoid oxygenases contribute to mammalian provitamin A metabolism."xsd:string |
| http://purl.uniprot.org/citations/24106281 | http://purl.uniprot.org/core/volume | "288"xsd:string |
| http://purl.uniprot.org/citations/24106281 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/24106281 |
| http://purl.uniprot.org/citations/24106281 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/24106281 |
| http://purl.uniprot.org/uniprot/#_B0YIX6-mappedCitation-24106281 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24106281 |
| http://purl.uniprot.org/uniprot/#_B0YIX7-mappedCitation-24106281 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24106281 |
| http://purl.uniprot.org/uniprot/#_B2RCV8-mappedCitation-24106281 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24106281 |
| http://purl.uniprot.org/uniprot/#_B2RUR5-mappedCitation-24106281 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24106281 |
| http://purl.uniprot.org/uniprot/#_B4DJC0-mappedCitation-24106281 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24106281 |
| http://purl.uniprot.org/uniprot/#_E9Q321-mappedCitation-24106281 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24106281 |
| http://purl.uniprot.org/uniprot/#_Q05AC0-mappedCitation-24106281 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24106281 |
| http://purl.uniprot.org/uniprot/#_Q3KNZ2-mappedCitation-24106281 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24106281 |