| http://purl.uniprot.org/citations/24115386 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24115386 | http://www.w3.org/2000/01/rdf-schema#comment | "p/CIP, also known as steroid receptor coactivator 3 (SRC-3)/Nuclear Receptor Coactivator 3 (NCoA3), is a transcriptional coactivator that binds liganded nuclear hormone receptors, as well as other transcription factors, and facilitates transcription through direct recruitment of accessory factors. We have found that p/CIP is highly expressed in undifferentiated mouse embryonic stem cells (mESCs) and is downregulated during differentiation. siRNA-mediated knockdown of p/CIP decreased transcript levels of Nanog, but not Oct4 or Sox2. Microarray expression analysis showed that Klf4, Tbx3, and Dax-1 are significantly downregulated in mESCs when p/CIP is knocked down. Subsequent chromatin immunoprecipitation (ChIP) analysis demonstrated that Tbx3, Klf4, and Dax-1 are direct transcriptional targets of p/CIP. Using the piggyBac transposition system, a mouse ESC line that expresses Flag-p/CIP in a doxycycline-dependent manner was generated. p/CIP overexpression increased the level of target genes and promoted the formation of undifferentiated colonies. Collectively, these results indicate that p/CIP contributes to the maintenance of ESC pluripotency through direct regulation of essential pluripotency genes. To better understand the mechanism by which p/CIP functions in ESC pluripotency, we integrated our ChIP and transcriptome data with published protein-protein interaction and promoter occupancy data to draft a p/CIP gene regulatory network. The p/CIP gene regulatory network identifies various feed-forward modules including one in which p/CIP activates members of the extended pluripotency network, demonstrating that p/CIP is a component of this extended network."xsd:string |
| http://purl.uniprot.org/citations/24115386 | http://purl.org/dc/terms/identifier | "doi:10.1002/stem.1564"xsd:string |
| http://purl.uniprot.org/citations/24115386 | http://purl.uniprot.org/core/author | "Chang W.Y."xsd:string |
| http://purl.uniprot.org/citations/24115386 | http://purl.uniprot.org/core/author | "Stanford W.L."xsd:string |
| http://purl.uniprot.org/citations/24115386 | http://purl.uniprot.org/core/author | "Torchia J."xsd:string |
| http://purl.uniprot.org/citations/24115386 | http://purl.uniprot.org/core/author | "Walker E."xsd:string |
| http://purl.uniprot.org/citations/24115386 | http://purl.uniprot.org/core/author | "Thillainadesan G."xsd:string |
| http://purl.uniprot.org/citations/24115386 | http://purl.uniprot.org/core/author | "Isovic M."xsd:string |
| http://purl.uniprot.org/citations/24115386 | http://purl.uniprot.org/core/author | "Manias J.L."xsd:string |
| http://purl.uniprot.org/citations/24115386 | http://purl.uniprot.org/core/author | "Chitilian J.M."xsd:string |
| http://purl.uniprot.org/citations/24115386 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24115386 | http://purl.uniprot.org/core/name | "Stem Cells"xsd:string |
| http://purl.uniprot.org/citations/24115386 | http://purl.uniprot.org/core/pages | "204-215"xsd:string |
| http://purl.uniprot.org/citations/24115386 | http://purl.uniprot.org/core/title | "Critical components of the pluripotency network are targets for the p300/CBP interacting protein (p/CIP) in embryonic stem cells."xsd:string |
| http://purl.uniprot.org/citations/24115386 | http://purl.uniprot.org/core/volume | "32"xsd:string |
| http://purl.uniprot.org/citations/24115386 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/24115386 |
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| http://purl.uniprot.org/uniprot/#_A2A468-mappedCitation-24115386 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24115386 |
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| http://purl.uniprot.org/uniprot/#_Q3UQL7-mappedCitation-24115386 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24115386 |