| http://purl.uniprot.org/citations/24123053 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24123053 | http://www.w3.org/2000/01/rdf-schema#comment | "Phospholipase C-η (PLCη) enzymes are a class of phosphatidylinositol 4,5-bisphosphate-hydrolyzing enzymes involved in intracellular signaling. PLCη2 can sense Ca(2+) (stimulated by ∼1 µM free Ca(2+) ) suggesting that it can amplify transient Ca(2+) signals. PLCη enzymes possess an EF-hand domain composed of two EF-loops; a canonical 12-residue loop (EF-loop 1) and a non-canonical 13-residue loop (EF-loop 2). Ca(2+) -binding to synthetic peptides corresponding to EF-loops 1 and 2 of PLCη2 and EF-loop 1 of calmodulin (as a control) was examined by 2D-[(1) H,(1) H] TOCSY NMR. Both PLCη2 EF-loop peptides bound Ca(2+) in a similar manner to that of the canonical calmodulin EF-loop 1, particularly at their N-terminus. A molecular model of the PLCη2 EF-hand domain, constructed based upon the structure of calmodulin, suggested both EF-loops may participate in Ca(2+) -binding. To determine whether the EF-hand is responsible for Ca(2+) -sensing, inositol phosphate accumulation was measured in COS7 cells transiently expressing wild-type or mutant PLCη2 proteins. Addition of 70 µM monensin (a Na(+) /H(+) antiporter that increases intracellular Ca(2+) ) induced a 4-to 7-fold increase in wild-type PLCη2 activity. In permeabilized cells, PLCη2 exhibited a ∼4-fold increase in activity in the presence of 1 µM free Ca(2+) . The D256A (EF-loop1) mutant exhibited a ∼10-fold reduction in Ca(2+) -sensitivity and was not activated by monensin, highlighting the involvement of EF-loop 1 in Ca(2+) -sensing. Involvement of EF-loop 2 was examined using D292A, H296A, Q297A, and E304A mutants. Interestingly, the monensin responses and Ca(2+) -sensitivities were largely unaffected by the mutations, indicating that the non-canonical EF-loop 2 is not involved in Ca(2+) -sensing."xsd:string |
| http://purl.uniprot.org/citations/24123053 | http://purl.org/dc/terms/identifier | "doi:10.1002/jcb.24690"xsd:string |
| http://purl.uniprot.org/citations/24123053 | http://purl.uniprot.org/core/author | "Lu J."xsd:string |
| http://purl.uniprot.org/citations/24123053 | http://purl.uniprot.org/core/author | "Schmid R."xsd:string |
| http://purl.uniprot.org/citations/24123053 | http://purl.uniprot.org/core/author | "Morgan K."xsd:string |
| http://purl.uniprot.org/citations/24123053 | http://purl.uniprot.org/core/author | "Blindauer C.A."xsd:string |
| http://purl.uniprot.org/citations/24123053 | http://purl.uniprot.org/core/author | "Stewart A.J."xsd:string |
| http://purl.uniprot.org/citations/24123053 | http://purl.uniprot.org/core/author | "Millar R.P."xsd:string |
| http://purl.uniprot.org/citations/24123053 | http://purl.uniprot.org/core/author | "Popovics P."xsd:string |
| http://purl.uniprot.org/citations/24123053 | http://purl.uniprot.org/core/author | "Nadia Kamil L."xsd:string |
| http://purl.uniprot.org/citations/24123053 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24123053 | http://purl.uniprot.org/core/name | "J Cell Biochem"xsd:string |
| http://purl.uniprot.org/citations/24123053 | http://purl.uniprot.org/core/pages | "557-565"xsd:string |
| http://purl.uniprot.org/citations/24123053 | http://purl.uniprot.org/core/title | "A canonical EF-loop directs Ca(2+) -sensitivity in phospholipase C-eta2."xsd:string |
| http://purl.uniprot.org/citations/24123053 | http://purl.uniprot.org/core/volume | "115"xsd:string |
| http://purl.uniprot.org/citations/24123053 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/24123053 |
| http://purl.uniprot.org/citations/24123053 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/24123053 |
| http://purl.uniprot.org/uniprot/#_Q4KWH8-mappedCitation-24123053 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24123053 |
| http://purl.uniprot.org/uniprot/Q4KWH8 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/24123053 |