| http://purl.uniprot.org/citations/24124444 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24124444 | http://www.w3.org/2000/01/rdf-schema#comment | "5-Fluorouracil (5-FU) is an anticancer drug and pyrimidine analogue. A problem in 5-FU therapy is acquired resistance to the drug. To find out more about the mechanisms of resistance, we screened a plasmid library in yeast for genes that confer 5-FU resistance when overexpressed. We cloned five genes: CPA1, CPA2, HMS1, HAM1 and YJL055W. CPA1 and CPA2 encode a carbamoyl phosphate synthase involved in arginine biosynthesis and HMS1 a helix-loop-helix transcription factor. Our results suggest that CPA1, CPA2, and HMS1 confer 5-FU resistance by stimulating pyrimidine biosynthesis. Thus, they are unable to confer 5-FU resistance in a ura2 mutant, and inhibit the uptake and incorporation into RNA of both uracil and 5-FU. In contrast, HAM1 and YJL055W confer 5-FU resistance in a ura2 mutant, and selectively inhibit incorporation into RNA of 5-FU but not uracil. HAM1 is the strongest resistance gene, but it partially depends on YJL055W for its function. This suggests that HAM1 and YJL055W function together in mediating resistance to 5-FU. Ham1p encodes an inosine triphosphate pyrophosphatase that has been implicated in resistance to purine analogues. Our results suggest that Ham1p could have a broader specificity that includes 5-FUTP and other pyrimidine analogoue triphosphates."xsd:string |
| http://purl.uniprot.org/citations/24124444 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0052094"xsd:string |
| http://purl.uniprot.org/citations/24124444 | http://purl.uniprot.org/core/author | "Muren E."xsd:string |
| http://purl.uniprot.org/citations/24124444 | http://purl.uniprot.org/core/author | "Ronne H."xsd:string |
| http://purl.uniprot.org/citations/24124444 | http://purl.uniprot.org/core/author | "Gustavsson M."xsd:string |
| http://purl.uniprot.org/citations/24124444 | http://purl.uniprot.org/core/author | "Carlsson M."xsd:string |
| http://purl.uniprot.org/citations/24124444 | http://purl.uniprot.org/core/author | "Hu G.Z."xsd:string |
| http://purl.uniprot.org/citations/24124444 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/24124444 | http://purl.uniprot.org/core/name | "PLoS One"xsd:string |
| http://purl.uniprot.org/citations/24124444 | http://purl.uniprot.org/core/pages | "e52094"xsd:string |
| http://purl.uniprot.org/citations/24124444 | http://purl.uniprot.org/core/title | "A Ham1p-dependent mechanism and modulation of the pyrimidine biosynthetic pathway can both confer resistance to 5-fluorouracil in yeast."xsd:string |
| http://purl.uniprot.org/citations/24124444 | http://purl.uniprot.org/core/volume | "8"xsd:string |
| http://purl.uniprot.org/citations/24124444 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/24124444 |
| http://purl.uniprot.org/citations/24124444 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/24124444 |
| http://purl.uniprot.org/uniprot/#_P20051-mappedCitation-24124444 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24124444 |
| http://purl.uniprot.org/uniprot/#_P22768-mappedCitation-24124444 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24124444 |
| http://purl.uniprot.org/uniprot/#_P28272-mappedCitation-24124444 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24124444 |
| http://purl.uniprot.org/uniprot/#_P13298-mappedCitation-24124444 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24124444 |
| http://purl.uniprot.org/uniprot/#_Q04728-mappedCitation-24124444 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24124444 |
| http://purl.uniprot.org/uniprot/#_P03965-mappedCitation-24124444 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24124444 |
| http://purl.uniprot.org/uniprot/#_P05150-mappedCitation-24124444 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24124444 |
| http://purl.uniprot.org/uniprot/#_P07258-mappedCitation-24124444 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24124444 |
| http://purl.uniprot.org/uniprot/#_P07259-mappedCitation-24124444 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24124444 |
| http://purl.uniprot.org/uniprot/#_P40360-mappedCitation-24124444 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24124444 |