| http://purl.uniprot.org/citations/24135698 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24135698 | http://www.w3.org/2000/01/rdf-schema#comment | "Chromatin immunoprecipitation (ChIP) is widely used for mapping DNA-protein interactions across eukaryotic genomes in cells, tissues or even whole organisms. Critical to this procedure is the efficient cross-linking of chromatin-associated proteins to DNA sequences that are in close proximity. Since the mid-nineties formaldehyde fixation has been the method of choice. However, some protein-DNA complexes cannot be successfully captured for ChIP using formaldehyde. One such formaldehyde refractory complex is the developmentally regulated insulator factor, Elba. Here we describe a new embryo fixation procedure using the bi-functional cross-linking reagents DSG (disuccinimidyl glutarate) and DSP (dithiobis[succinimidyl propionate). We show that unlike standard formaldehyde fixation protocols, it is possible to capture Elba association with insulator elements in 2-5 h embryos using this new cross-linking procedure. We show that this new cross-linking procedure can also be applied to localize nuclear proteins that are amenable to ChIP using standard formaldehyde cross-linking protocols, and that in the cases tested the enrichment was generally superior to that achieved using formaldehyde cross-linking."xsd:string |
| http://purl.uniprot.org/citations/24135698 | http://purl.org/dc/terms/identifier | "doi:10.4161/fly.26805"xsd:string |
| http://purl.uniprot.org/citations/24135698 | http://purl.uniprot.org/core/author | "Aoki T."xsd:string |
| http://purl.uniprot.org/citations/24135698 | http://purl.uniprot.org/core/author | "Dai Q."xsd:string |
| http://purl.uniprot.org/citations/24135698 | http://purl.uniprot.org/core/author | "Lai E.C."xsd:string |
| http://purl.uniprot.org/citations/24135698 | http://purl.uniprot.org/core/author | "Schedl P."xsd:string |
| http://purl.uniprot.org/citations/24135698 | http://purl.uniprot.org/core/author | "Wolle D."xsd:string |
| http://purl.uniprot.org/citations/24135698 | http://purl.uniprot.org/core/author | "Preger-Ben Noon E."xsd:string |
| http://purl.uniprot.org/citations/24135698 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24135698 | http://purl.uniprot.org/core/name | "Fly (Austin)"xsd:string |
| http://purl.uniprot.org/citations/24135698 | http://purl.uniprot.org/core/pages | "43-51"xsd:string |
| http://purl.uniprot.org/citations/24135698 | http://purl.uniprot.org/core/title | "Bi-functional cross-linking reagents efficiently capture protein-DNA complexes in Drosophila embryos."xsd:string |
| http://purl.uniprot.org/citations/24135698 | http://purl.uniprot.org/core/volume | "8"xsd:string |
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| http://purl.uniprot.org/citations/24135698 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/24135698 |
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