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http://purl.uniprot.org/citations/24151602http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/24151602http://www.w3.org/2000/01/rdf-schema#comment"

Purpose

To evaluate the effect of a traditional Chinese medicine, Rheum Polysaccharide (RP), on the in vitro production of tumor necrosis factor alpha (TNF- α ) and interleukin-10 (IL-10) by lipopolysaccharide-(LPS-)stimulated human monocytes from HLA-B27 associated acute anterior uveitis patients of convalescence stage.

Method

PBMC samples were isolated from 10 HLA-B27 associated acute anterior uveitis, incubated, respectively, and divided into 4 groups as follows: (1) controls, PBS was added in final concentration of 1 mg·L⁻¹, (2) stimulated by LPS, LPS was added in final concentration of 1 mg·L⁻¹, (3) stimulated by LPS + HTA125, 30 minutes before the adding of LPS in final concentration of 1 mg·L⁻¹, the final concentration of 5 mg·L⁻¹ of the HTA125 was added, and (4) stimulated by LPS + RP, 30 minutes before the adding of LPS in final concentration 1 mg·L⁻¹, the final concentration 100 mg·L⁻¹ of the RP was added. Supernatants were used to quantify the amounts of TNF-α and IL-10 released in time course using enzyme-linked immunosorbent assay (ELISA).

Result

After stimulated by lps, the concentrations of TNF-α and IL-10 in culture supernatants of patients are significantly higher than control group at all time points (P < 0.01). Blockage of TLR-4 by HTA125 can decrease the production of TNF-α and IL-10 compared with lps group (P < 0.01, except at 4 h group of IL-10). Concentration of TNF-α and IL-10 also decreases in the LPS + RP group (P < 0.01) but not so significantly as in the LPS + HTA125 group.

Conclusion

As anti-TLR4 monoclonal antibodies, rheum Polysaccharide can also inhibit the secretion of cytokines produced by monocytes from HLA-B27 positive AAU patients of convalescence stage."xsd:string
http://purl.uniprot.org/citations/24151602http://purl.org/dc/terms/identifier"doi:10.1155/2013/431232"xsd:string
http://purl.uniprot.org/citations/24151602http://purl.uniprot.org/core/author"Hu X."xsd:string
http://purl.uniprot.org/citations/24151602http://purl.uniprot.org/core/author"Li Z."xsd:string
http://purl.uniprot.org/citations/24151602http://purl.uniprot.org/core/author"Liu X."xsd:string
http://purl.uniprot.org/citations/24151602http://purl.uniprot.org/core/author"Lu H."xsd:string
http://purl.uniprot.org/citations/24151602http://purl.uniprot.org/core/author"Zhang X."xsd:string
http://purl.uniprot.org/citations/24151602http://purl.uniprot.org/core/date"2013"xsd:gYear
http://purl.uniprot.org/citations/24151602http://purl.uniprot.org/core/name"Biomed Res Int"xsd:string
http://purl.uniprot.org/citations/24151602http://purl.uniprot.org/core/pages"431232"xsd:string
http://purl.uniprot.org/citations/24151602http://purl.uniprot.org/core/title"Role of Rheum Polysaccharide in the cytokines produced by peripheral blood monocytes in TLR4 mediated HLA-B27 associated AAU."xsd:string
http://purl.uniprot.org/citations/24151602http://purl.uniprot.org/core/volume"2013"xsd:string
http://purl.uniprot.org/citations/24151602http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/24151602
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http://purl.uniprot.org/uniprot/#_A0A068B6T8-mappedCitation-24151602http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/24151602
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