| http://purl.uniprot.org/citations/24168112 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24168112 | http://www.w3.org/2000/01/rdf-schema#comment | "Galanin and its receptors, GALR1 and GALR2, are known tumor suppressors and potential therapeutic targets in head and neck squamous cell carcinoma (HNSCC). Previously, we demonstrated that, in GALR1-expressing HNSCC cells, the addition of galanin suppressed tumor proliferation via upregulation of ERK1/2 and cyclin-dependent kinase inhibitors, whereas, in GALR2-expressing cells, the addition of galanin not only suppressed proliferation, but also induced apoptosis. In this study, we first transduced HEp-2 and KB cell lines using a recombinant adeno-associated virus (rAAV)-green fluorescent protein (GFP) vector and confirmed a high GFP expression rate (>90%) in both cell lines at the standard vector dose. Next, we demonstrated that GALR2 expression in the presence of galanin suppressed cell viability to 40-60% after 72 h in both cell lines. Additionally, the annexin V-positive rate and sub-G0/G1 phase population were significantly elevated in HEp-2 cells (mock vs GALR2: 12.3 vs 25.0% (P < 0.01) and 9.1 vs 32.0% (P < 0.05), respectively) after 48 h. These changes were also observed in KB cells, although to a lesser extent. Furthermore, in HEp-2 cells, GALR2-mediated apoptosis was caspase-independent, involving downregulation of ERK1/2, followed by induction of the pro-apoptotic Bcl-2 protein, Bim. These results illustrate that transient GALR2 expression in the presence of galanin induces apoptosis via diverse pathways and serves as a platform for suicide gene therapy against HNSCC."xsd:string |
| http://purl.uniprot.org/citations/24168112 | http://purl.org/dc/terms/identifier | "doi:10.1111/cas.12315"xsd:string |
| http://purl.uniprot.org/citations/24168112 | http://purl.uniprot.org/core/author | "Ozawa K."xsd:string |
| http://purl.uniprot.org/citations/24168112 | http://purl.uniprot.org/core/author | "Mizukami H."xsd:string |
| http://purl.uniprot.org/citations/24168112 | http://purl.uniprot.org/core/author | "Suzuki M."xsd:string |
| http://purl.uniprot.org/citations/24168112 | http://purl.uniprot.org/core/author | "Uehara T."xsd:string |
| http://purl.uniprot.org/citations/24168112 | http://purl.uniprot.org/core/author | "Ichimura K."xsd:string |
| http://purl.uniprot.org/citations/24168112 | http://purl.uniprot.org/core/author | "Tsukahara T."xsd:string |
| http://purl.uniprot.org/citations/24168112 | http://purl.uniprot.org/core/author | "Kanazawa T."xsd:string |
| http://purl.uniprot.org/citations/24168112 | http://purl.uniprot.org/core/author | "Carey T.E."xsd:string |
| http://purl.uniprot.org/citations/24168112 | http://purl.uniprot.org/core/author | "Misawa K."xsd:string |
| http://purl.uniprot.org/citations/24168112 | http://purl.uniprot.org/core/author | "Kume A."xsd:string |
| http://purl.uniprot.org/citations/24168112 | http://purl.uniprot.org/core/author | "Urabe M."xsd:string |
| http://purl.uniprot.org/citations/24168112 | http://purl.uniprot.org/core/author | "Uchibori R."xsd:string |
| http://purl.uniprot.org/citations/24168112 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24168112 | http://purl.uniprot.org/core/name | "Cancer Sci"xsd:string |
| http://purl.uniprot.org/citations/24168112 | http://purl.uniprot.org/core/pages | "72-80"xsd:string |
| http://purl.uniprot.org/citations/24168112 | http://purl.uniprot.org/core/title | "Novel anti-tumor mechanism of galanin receptor type 2 in head and neck squamous cell carcinoma cells."xsd:string |
| http://purl.uniprot.org/citations/24168112 | http://purl.uniprot.org/core/volume | "105"xsd:string |
| http://purl.uniprot.org/citations/24168112 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/24168112 |
| http://purl.uniprot.org/citations/24168112 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/24168112 |
| http://purl.uniprot.org/uniprot/#_O43603-mappedCitation-24168112 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24168112 |
| http://purl.uniprot.org/uniprot/O43603 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/24168112 |