| http://purl.uniprot.org/citations/24196533 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24196533 | http://www.w3.org/2000/01/rdf-schema#comment | "Protein phosphatase 1 (PP1) and Ca2+/calmodulin-dependent protein kinase δ (CaMKIIδ) are upregulated in heart disorders. Alternative splicing factor (ASF), a major splice factor for CaMKIIδ splicing, can be regulated by both protein kinase and phosphatase. Here we determine the role of PP1 isoforms in ASF-mediated splicing of CaMKIIδ in cells. We found that 1) PP1γ, but not α or β isoform, enhanced the splicing of CaMKIIδ in HEK293T cells; 2) PP1γ promoted the function of ASF, evidenced by the existence of ASF-PP1γ association as well as the PP1γ overexpression- or silencing-mediated change in CaMKIIδ splicing in ASF-transfected HEK293T cells; 3) CaMKIIδ splicing was promoted by overexpression of PP1γ and impaired by application of PP1 inhibitor 1 (I1PP1) or pharmacological inhibitor tautomycetin in primary cardiomyocytes; 4) CaMKIIδ splicing and enhancement of ASF-PP1γ association induced by oxygen-glucose deprivation followed by reperfusion (OGD/R) were potentiated by overexpression of PP1γ and suppressed by inhibition of PP1γ with I1PP1 or tautomycetin in primary cardiomyocytes; 5) functionally, overexpression and inhibition of PP1γ, respectively, potentiated or suppressed the apoptosis and Bax/Bcl-2 ratio, which were associated with the enhanced activity of CaMKII in OGD/R-stimulated cardiomyocytes; and 6) CaMKII was required for the OGD/R induced- and PP1γ exacerbated-apoptosis of cardiomyocytes, evidenced by a specific inhibitor of CaMKII KN93, but not its structural analog KN92, attenuating the apoptosis and Bax/Bcl-2 ratio in OGD/R and PP1γ-treated cells. In conclusion, our results show that PP1γ promotes the alternative splicing of CaMKIIδ through its interacting with ASF, exacerbating OGD/R-triggered apoptosis in primary cardiomyocytes."xsd:string |
| http://purl.uniprot.org/citations/24196533 | http://purl.org/dc/terms/identifier | "doi:10.1152/ajpcell.00145.2013"xsd:string |
| http://purl.uniprot.org/citations/24196533 | http://purl.uniprot.org/core/author | "Chen X."xsd:string |
| http://purl.uniprot.org/citations/24196533 | http://purl.uniprot.org/core/author | "Liao R."xsd:string |
| http://purl.uniprot.org/citations/24196533 | http://purl.uniprot.org/core/author | "Huang C."xsd:string |
| http://purl.uniprot.org/citations/24196533 | http://purl.uniprot.org/core/author | "Tong L."xsd:string |
| http://purl.uniprot.org/citations/24196533 | http://purl.uniprot.org/core/author | "Wang Y."xsd:string |
| http://purl.uniprot.org/citations/24196533 | http://purl.uniprot.org/core/author | "Wang J."xsd:string |
| http://purl.uniprot.org/citations/24196533 | http://purl.uniprot.org/core/author | "Zhang W."xsd:string |
| http://purl.uniprot.org/citations/24196533 | http://purl.uniprot.org/core/author | "Zhu W."xsd:string |
| http://purl.uniprot.org/citations/24196533 | http://purl.uniprot.org/core/author | "Cao W."xsd:string |
| http://purl.uniprot.org/citations/24196533 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24196533 | http://purl.uniprot.org/core/name | "Am J Physiol Cell Physiol"xsd:string |
| http://purl.uniprot.org/citations/24196533 | http://purl.uniprot.org/core/pages | "C167-77"xsd:string |
| http://purl.uniprot.org/citations/24196533 | http://purl.uniprot.org/core/title | "PP1gamma functionally augments the alternative splicing of CaMKIIdelta through interaction with ASF."xsd:string |
| http://purl.uniprot.org/citations/24196533 | http://purl.uniprot.org/core/volume | "306"xsd:string |
| http://purl.uniprot.org/citations/24196533 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/24196533 |
| http://purl.uniprot.org/citations/24196533 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/24196533 |
| http://purl.uniprot.org/uniprot/#_E9PBG7-mappedCitation-24196533 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24196533 |
| http://purl.uniprot.org/uniprot/#_E9PF82-mappedCitation-24196533 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24196533 |
| http://purl.uniprot.org/uniprot/#_A0A8V8TPJ8-mappedCitation-24196533 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24196533 |
| http://purl.uniprot.org/uniprot/#_A0A8V8TPY4-mappedCitation-24196533 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24196533 |
| http://purl.uniprot.org/uniprot/#_A0A8V8TN60-mappedCitation-24196533 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24196533 |
| http://purl.uniprot.org/uniprot/#_A0A8V8TN65-mappedCitation-24196533 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24196533 |