| http://purl.uniprot.org/citations/24202351 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24202351 | http://www.w3.org/2000/01/rdf-schema#comment | "Two semidwarfism-related proteins, SRP-1 and SRP-2, were detected as major spots in a long-culm rice cultivar, Norin 29 and its semidwarf near-isogenic line, SC-TN1, respectively, by two-dimensional gel electrophoresis (2D-PAGE). The testcross showed that SRP-1 and SRP-2 are controlled by codominant alleles, Srp-1 and Srp-2, respectively, at a single locus Srp. This locus was considered to be closely linked with the semidwarfing gene locus sd-1. SRP-1 and SRP-2 were separated by 2D-PAGE, electroblotted onto a polyvinylidene difluoride membrane, and sequenced by a gas-phase protein sequencer. The N-terminal amino acid sequences, however, could not be determined due to the blockage of the N-terminals of these proteins. After removal of the N-terminal residue with pyroglutamyl peptidase given to the membrane, the amino acid sequence in the N-terminal region was determined. The N-terminal and internal amino acid sequences of SRP-1 and SRP-2 were highly homologous with those of the glutelin α-subunits of seed endosperm storage protein, which were deduced by the cDNA sequences. In the seed endosperms of Norin 29 and SC-TN1, a total of eight glutelin α-subunits was identified by 2D-PAGE. The amino acid sequences in the N-terminal and internal regions of these proteins were determined. This experiment confirmed that SRP-1 and SRP-2 are almost identical in structure with the glutelin α5a- and α5b-subunits, respectively, which were identified in several organs such as endosperms, embryos, and leaves, unlike the other glutelin α-subunits."xsd:string |
| http://purl.uniprot.org/citations/24202351 | http://purl.org/dc/terms/identifier | "doi:10.1007/bf00226244"xsd:string |
| http://purl.uniprot.org/citations/24202351 | http://purl.uniprot.org/core/author | "Hirano H."xsd:string |
| http://purl.uniprot.org/citations/24202351 | http://purl.uniprot.org/core/author | "Nakamura A."xsd:string |
| http://purl.uniprot.org/citations/24202351 | http://purl.uniprot.org/core/author | "Tsunasawa S."xsd:string |
| http://purl.uniprot.org/citations/24202351 | http://purl.uniprot.org/core/author | "Sakiyama F."xsd:string |
| http://purl.uniprot.org/citations/24202351 | http://purl.uniprot.org/core/author | "Komatsu S."xsd:string |
| http://purl.uniprot.org/citations/24202351 | http://purl.uniprot.org/core/author | "Kajiwara H."xsd:string |
| http://purl.uniprot.org/citations/24202351 | http://purl.uniprot.org/core/author | "Kikuchi F."xsd:string |
| http://purl.uniprot.org/citations/24202351 | http://purl.uniprot.org/core/date | "1991"xsd:gYear |
| http://purl.uniprot.org/citations/24202351 | http://purl.uniprot.org/core/name | "Theor Appl Genet"xsd:string |
| http://purl.uniprot.org/citations/24202351 | http://purl.uniprot.org/core/pages | "153-158"xsd:string |
| http://purl.uniprot.org/citations/24202351 | http://purl.uniprot.org/core/title | "Structural homology between semidwarfism-related proteins and glutelin seed protein in rice (Oryza sativa L.)."xsd:string |
| http://purl.uniprot.org/citations/24202351 | http://purl.uniprot.org/core/volume | "83"xsd:string |
| http://purl.uniprot.org/citations/24202351 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/24202351 |
| http://purl.uniprot.org/citations/24202351 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/24202351 |
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