| http://purl.uniprot.org/citations/24203998 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24203998 | http://www.w3.org/2000/01/rdf-schema#comment | "Pauci-immune focal necrotizing GN (piFNGN) is usually associated with ANCAs that are thought to be pathogenic. However, 10%-15% of patients are ANCA negative and the cause of their injury is unknown. We previously reported a high frequency of autoantibodies to human lysosome-associated membrane protein-2 (hLAMP-2) in ANCA-associated piFNGN, and have now investigated whether the same is true in ANCA-negative patients. Of 11 patients, 8 (73%) had anti-hLAMP-2 antibodies detected by ELISA and confirmed by immunoblotting and indirect immunofluorescence. The autoantibodies from all 8 patients bound to native LAMP-2 purified from human glomeruli and recombinant hLAMP-2 expressed in ldlD cells, both with molecular masses of 110 kD. However, in contrast to anti-LAMP-2 antibodies from ANCA-positive patients, these antibodies from ANCA-negative patients failed to bind the more complexly glycosylated native neutrophil hLAMP-2 (190 kD). Treatment with the deglycosylating enzyme, endo-β-galactosidase, reduced the mass of neutrophil hLAMP-2 to 110 kD and enabled autoantibody binding. Similarly, pretreating neutrophils with endo-β-galactosidase or neuraminidase converted ANCA assay results from negative to positive. Finally, IgG from LAMP-2-positive ANCA-negative patients bound specifically to normal human kidney sections and to human glomerular endothelial cells in culture. In conclusion, in patients with ANCA-negative piFNGN, we have identified autoantibodies to hLAMP-2 that bind native glomerular but not neutrophil hLAMP-2, suggesting a role in pathogenesis."xsd:string |
| http://purl.uniprot.org/citations/24203998 | http://purl.org/dc/terms/identifier | "doi:10.1681/asn.2013030320"xsd:string |
| http://purl.uniprot.org/citations/24203998 | http://purl.uniprot.org/core/author | "Brown M."xsd:string |
| http://purl.uniprot.org/citations/24203998 | http://purl.uniprot.org/core/author | "Peschel A."xsd:string |
| http://purl.uniprot.org/citations/24203998 | http://purl.uniprot.org/core/author | "Kain R."xsd:string |
| http://purl.uniprot.org/citations/24203998 | http://purl.uniprot.org/core/author | "Basu N."xsd:string |
| http://purl.uniprot.org/citations/24203998 | http://purl.uniprot.org/core/author | "Brandes R."xsd:string |
| http://purl.uniprot.org/citations/24203998 | http://purl.uniprot.org/core/author | "Rees A.J."xsd:string |
| http://purl.uniprot.org/citations/24203998 | http://purl.uniprot.org/core/author | "Benharkou A."xsd:string |
| http://purl.uniprot.org/citations/24203998 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24203998 | http://purl.uniprot.org/core/name | "J Am Soc Nephrol"xsd:string |
| http://purl.uniprot.org/citations/24203998 | http://purl.uniprot.org/core/pages | "455-463"xsd:string |
| http://purl.uniprot.org/citations/24203998 | http://purl.uniprot.org/core/title | "Autoantibodies to hLAMP-2 in ANCA-negative pauci-immune focal necrotizing GN."xsd:string |
| http://purl.uniprot.org/citations/24203998 | http://purl.uniprot.org/core/volume | "25"xsd:string |
| http://purl.uniprot.org/citations/24203998 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/24203998 |
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