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http://purl.uniprot.org/citations/24222120http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/24222120http://www.w3.org/2000/01/rdf-schema#comment"

Background

The purpose of this study was to identify the DNA methylation status of the mitogen-activated protein kinase (MAPK) signal-inhibiting genes dual-specificity phosphatase 4 (DUSP4) and 6 (DUSP6); and serpin peptidase inhibitor A member 5 (SERPINA5) in thyroid cancer.

Materials and methods

Using 76 papillary thyroid cancer(PTC) tissues and three thyroid cancer cell lines (TPC1, WRO82-1 and XTC), the expression of three genes and DNA methylation were determined by reverse transcription-PCR and methylation-specific PCR.

Results

In all cell lines, the expression of DUSP4 and DUSP6 increased; the corresponding gene promoters were unmethylated. However, SERPINA5 gene expression decreased and SERPINA5 DNA was methylated in the TPC1 cell line. With the de-methylating agent 5'-aza-2'-deoxycytidine, SERPINA5 gene expression was restored. In 82.9% of PTC tissues (63/76), the SERPINA5 DNA promoter was methylated, which was associated with a higher v-raf murine sarcoma viral oncogene homolog B1(BRAF) mutation rate in PTC tissues based on multivariate regression (odds ratio=3.573; 95% confidence interval=1.122-11.379; p=0.031).

Conclusion

The expression of the MAPK signal-inhibiting gene SERPINA5 decreased in the TPC1 cell line, SERPINA5 expression was regulated by DNA methylation, which was associated with a higher BRAF mutation rate in PTC."xsd:string
http://purl.uniprot.org/citations/24222120http://purl.uniprot.org/core/author"Kim S.W."xsd:string
http://purl.uniprot.org/citations/24222120http://purl.uniprot.org/core/author"Kang H.S."xsd:string
http://purl.uniprot.org/citations/24222120http://purl.uniprot.org/core/author"Lee E.K."xsd:string
http://purl.uniprot.org/citations/24222120http://purl.uniprot.org/core/author"Park M.J."xsd:string
http://purl.uniprot.org/citations/24222120http://purl.uniprot.org/core/author"Chung K.W."xsd:string
http://purl.uniprot.org/citations/24222120http://purl.uniprot.org/core/author"Yang S.K."xsd:string
http://purl.uniprot.org/citations/24222120http://purl.uniprot.org/core/author"Min H.S."xsd:string
http://purl.uniprot.org/citations/24222120http://purl.uniprot.org/core/date"2013"xsd:gYear
http://purl.uniprot.org/citations/24222120http://purl.uniprot.org/core/name"Anticancer Res"xsd:string
http://purl.uniprot.org/citations/24222120http://purl.uniprot.org/core/pages"4833-4839"xsd:string
http://purl.uniprot.org/citations/24222120http://purl.uniprot.org/core/title"DNA methylation of MAPK signal-inhibiting genes in papillary thyroid carcinoma."xsd:string
http://purl.uniprot.org/citations/24222120http://purl.uniprot.org/core/volume"33"xsd:string
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