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http://purl.uniprot.org/citations/24240322http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/24240322http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/24240322http://www.w3.org/2000/01/rdf-schema#comment"We present a liquid chromatography-mass spectrometry (LC-MS)-based method permitting unbiased (gene prediction-independent) genome-wide discovery of protein-coding loci in higher eukaryotes. Using high-resolution isoelectric focusing (HiRIEF) at the peptide level in the 3.7-5.0 pH range and accurate peptide isoelectric point (pI) prediction, we probed the six-reading-frame translation of the human and mouse genomes and identified 98 and 52 previously undiscovered protein-coding loci, respectively. The method also enabled deep proteome coverage, identifying 13,078 human and 10,637 mouse proteins."xsd:string
http://purl.uniprot.org/citations/24240322http://purl.org/dc/terms/identifier"doi:10.1038/nmeth.2732"xsd:string
http://purl.uniprot.org/citations/24240322http://purl.org/dc/terms/identifier"doi:10.1038/nmeth.2732"xsd:string
http://purl.uniprot.org/citations/24240322http://purl.uniprot.org/core/author"Branca R.M."xsd:string
http://purl.uniprot.org/citations/24240322http://purl.uniprot.org/core/author"Branca R.M."xsd:string
http://purl.uniprot.org/citations/24240322http://purl.uniprot.org/core/author"Lehtioe J."xsd:string
http://purl.uniprot.org/citations/24240322http://purl.uniprot.org/core/author"Lehtioe J."xsd:string
http://purl.uniprot.org/citations/24240322http://purl.uniprot.org/core/author"Johansson H.J."xsd:string
http://purl.uniprot.org/citations/24240322http://purl.uniprot.org/core/author"Johansson H.J."xsd:string
http://purl.uniprot.org/citations/24240322http://purl.uniprot.org/core/author"Huss M."xsd:string
http://purl.uniprot.org/citations/24240322http://purl.uniprot.org/core/author"Huss M."xsd:string
http://purl.uniprot.org/citations/24240322http://purl.uniprot.org/core/author"Forshed J."xsd:string
http://purl.uniprot.org/citations/24240322http://purl.uniprot.org/core/author"Forshed J."xsd:string
http://purl.uniprot.org/citations/24240322http://purl.uniprot.org/core/author"Granholm V."xsd:string
http://purl.uniprot.org/citations/24240322http://purl.uniprot.org/core/author"Granholm V."xsd:string
http://purl.uniprot.org/citations/24240322http://purl.uniprot.org/core/author"Kaell L."xsd:string
http://purl.uniprot.org/citations/24240322http://purl.uniprot.org/core/author"Kaell L."xsd:string
http://purl.uniprot.org/citations/24240322http://purl.uniprot.org/core/author"Orre L.M."xsd:string
http://purl.uniprot.org/citations/24240322http://purl.uniprot.org/core/author"Orre L.M."xsd:string
http://purl.uniprot.org/citations/24240322http://purl.uniprot.org/core/author"Perez-Bercoff A."xsd:string
http://purl.uniprot.org/citations/24240322http://purl.uniprot.org/core/author"Perez-Bercoff A."xsd:string
http://purl.uniprot.org/citations/24240322http://purl.uniprot.org/core/date"2014"xsd:gYear
http://purl.uniprot.org/citations/24240322http://purl.uniprot.org/core/date"2014"xsd:gYear