| http://purl.uniprot.org/citations/24257752 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24257752 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24257752 | http://www.w3.org/2000/01/rdf-schema#comment | "Bacterial toxin-antitoxin (TA) systems regulate key cellular processes to promote cell survival during periods of stress. During steady-state cell growth, antitoxins typically interact with their cognate toxins to inhibit activity presumably by preventing substrate recognition. We solved two x-ray crystal structures of the Proteus vulgaris tetrameric HigB-(HigA)2-HigB TA complex and found that, unlike most other TA systems, the antitoxin HigA makes minimal interactions with toxin HigB. HigB adopts a RelE family tertiary fold containing a highly conserved concave surface where we predict its active site is located. HigA does not cover the solvent-exposed HigB active site, suggesting that, in general, toxin inhibition is not solely mediated by active site hindrance by its antitoxin. Each HigA monomer contains a helix-turn-helix motif that binds to its own DNA operator to repress transcription during normal cellular growth. This is distinct from antitoxins belonging to other superfamilies that typically only form DNA-binding motifs upon dimerization. We further show that disruption of the HigB-(HigA)2-HigB tetramer to a HigBA heterodimer ablates operator binding. Taken together, our biochemical and structural studies elucidate the novel molecular details of the HigBA TA system."xsd:string |
| http://purl.uniprot.org/citations/24257752 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m113.512095"xsd:string |
| http://purl.uniprot.org/citations/24257752 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m113.512095"xsd:string |
| http://purl.uniprot.org/citations/24257752 | http://purl.uniprot.org/core/author | "Marquez J."xsd:string |
| http://purl.uniprot.org/citations/24257752 | http://purl.uniprot.org/core/author | "Marquez J."xsd:string |
| http://purl.uniprot.org/citations/24257752 | http://purl.uniprot.org/core/author | "Dunham C.M."xsd:string |
| http://purl.uniprot.org/citations/24257752 | http://purl.uniprot.org/core/author | "Dunham C.M."xsd:string |
| http://purl.uniprot.org/citations/24257752 | http://purl.uniprot.org/core/author | "Maehigashi T."xsd:string |
| http://purl.uniprot.org/citations/24257752 | http://purl.uniprot.org/core/author | "Maehigashi T."xsd:string |
| http://purl.uniprot.org/citations/24257752 | http://purl.uniprot.org/core/author | "Miles S.J."xsd:string |
| http://purl.uniprot.org/citations/24257752 | http://purl.uniprot.org/core/author | "Miles S.J."xsd:string |
| http://purl.uniprot.org/citations/24257752 | http://purl.uniprot.org/core/author | "Cho S.E."xsd:string |
| http://purl.uniprot.org/citations/24257752 | http://purl.uniprot.org/core/author | "Cho S.E."xsd:string |
| http://purl.uniprot.org/citations/24257752 | http://purl.uniprot.org/core/author | "Erdman R."xsd:string |
| http://purl.uniprot.org/citations/24257752 | http://purl.uniprot.org/core/author | "Erdman R."xsd:string |
| http://purl.uniprot.org/citations/24257752 | http://purl.uniprot.org/core/author | "Schureck M.A."xsd:string |
| http://purl.uniprot.org/citations/24257752 | http://purl.uniprot.org/core/author | "Schureck M.A."xsd:string |
| http://purl.uniprot.org/citations/24257752 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24257752 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24257752 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/24257752 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/24257752 | http://purl.uniprot.org/core/pages | "1060-1070"xsd:string |
| http://purl.uniprot.org/citations/24257752 | http://purl.uniprot.org/core/pages | "1060-1070"xsd:string |