| http://purl.uniprot.org/citations/24258276 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24258276 | http://www.w3.org/2000/01/rdf-schema#comment | "Messenger RNA translation is regulated by RNA-binding proteins and small non-coding RNAs called microRNAs. Even though we know the majority of RNA-binding proteins and microRNAs that regulate messenger RNA expression, evidence of interactions between the two remain elusive. The role of the RNA-binding protein GLD-1 as a translational repressor is well studied during Caenorhabditis elegans germline development and maintenance. Possible functions of GLD-1 during somatic development and the mechanism of how GLD-1 acts as a translational repressor are not known. Its human homologue, quaking (QKI), is essential for embryonic development. Here, we report that the RNA-binding protein GLD-1 in C. elegans affects multiple microRNA pathways and interacts with proteins required for microRNA function. Using genome-wide RNAi screening, we found that nhl-2 and vig-1, two known modulators of miRNA function, genetically interact with GLD-1. gld-1 mutations enhance multiple phenotypes conferred by mir-35 and let-7 family mutants during somatic development. We used stable isotope labelling with amino acids in cell culture to globally analyse the changes in the proteome conferred by let-7 and gld-1 during animal development. We identified the histone mRNA-binding protein CDL-1 to be, in part, responsible for the phenotypes observed in let-7 and gld-1 mutants. The link between GLD-1 and miRNA-mediated gene regulation is further supported by its biochemical interaction with ALG-1, CGH-1 and PAB-1, proteins implicated in miRNA regulation. Overall, we have uncovered genetic and biochemical interactions between GLD-1 and miRNA pathways."xsd:string |
| http://purl.uniprot.org/citations/24258276 | http://purl.org/dc/terms/identifier | "doi:10.1098/rsob.130151"xsd:string |
| http://purl.uniprot.org/citations/24258276 | http://purl.uniprot.org/core/author | "Craig A."xsd:string |
| http://purl.uniprot.org/citations/24258276 | http://purl.uniprot.org/core/author | "Larance M."xsd:string |
| http://purl.uniprot.org/citations/24258276 | http://purl.uniprot.org/core/author | "Gartner A."xsd:string |
| http://purl.uniprot.org/citations/24258276 | http://purl.uniprot.org/core/author | "Pourkarimi E."xsd:string |
| http://purl.uniprot.org/citations/24258276 | http://purl.uniprot.org/core/author | "Lamond A."xsd:string |
| http://purl.uniprot.org/citations/24258276 | http://purl.uniprot.org/core/author | "Akay A."xsd:string |
| http://purl.uniprot.org/citations/24258276 | http://purl.uniprot.org/core/author | "Miska E."xsd:string |
| http://purl.uniprot.org/citations/24258276 | http://purl.uniprot.org/core/author | "Wright J.E."xsd:string |
| http://purl.uniprot.org/citations/24258276 | http://purl.uniprot.org/core/author | "Lehrbach N."xsd:string |
| http://purl.uniprot.org/citations/24258276 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/24258276 | http://purl.uniprot.org/core/name | "Open Biol"xsd:string |
| http://purl.uniprot.org/citations/24258276 | http://purl.uniprot.org/core/pages | "130151"xsd:string |
| http://purl.uniprot.org/citations/24258276 | http://purl.uniprot.org/core/title | "RNA-binding protein GLD-1/quaking genetically interacts with the mir-35 and the let-7 miRNA pathways in Caenorhabditis elegans."xsd:string |
| http://purl.uniprot.org/citations/24258276 | http://purl.uniprot.org/core/volume | "3"xsd:string |
| http://purl.uniprot.org/citations/24258276 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/24258276 |
| http://purl.uniprot.org/citations/24258276 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/24258276 |
| http://purl.uniprot.org/uniprot/#_A0A131MCL1-mappedCitation-24258276 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24258276 |
| http://purl.uniprot.org/uniprot/#_D4YW53-mappedCitation-24258276 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24258276 |
| http://purl.uniprot.org/uniprot/#_P11141-mappedCitation-24258276 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24258276 |
| http://purl.uniprot.org/uniprot/#_A0A486WX07-mappedCitation-24258276 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24258276 |
| http://purl.uniprot.org/uniprot/#_A0A5E4LXR0-mappedCitation-24258276 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24258276 |
| http://purl.uniprot.org/uniprot/#_A0A131MDC6-mappedCitation-24258276 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24258276 |