| http://purl.uniprot.org/citations/24287187 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24287187 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24287187 | http://www.w3.org/2000/01/rdf-schema#comment | "A large number of retaining glycosidases catalyze both hydrolysis and transglycosylation reactions, but little is known about what determines the balance between these two activities (transglycosylation/hydrolysis ratio). We previously obtained by directed evolution the mutants F401S and N282T of Thermus thermophilus β-glycosidase (Ttβ-gly, glycoside hydrolase family 1 (GH1)), which display a higher transglycosylation/hydrolysis ratio than the wild-type enzyme. In order to find the cause of these activity modifications, and thereby set up a generic method for easily obtaining transglycosidases from glycosidases, we determined their X-ray structure. No major structural changes could be observed which could help to rationalize the mutagenesis of glycosidases into transglycosidases. However, as these mutations are highly conserved in GH1 β-glycosidases and are located around the -1 site, we pursued the isolation of new transglycosidases by targeting highly conserved amino acids located around the active site. Thus, by single-point mutagenesis on Ttβ-gly, we created four new mutants that exhibit improved synthetic activity, producing disaccharides in yields of 68-90% against only 36% when native Ttβ-gly was used. As all of the chosen positions were well conserved among GH1 enzymes, this approach is most probably a general route to convert GH1 glycosidases into transglycosidases."xsd:string |
| http://purl.uniprot.org/citations/24287187 | http://purl.org/dc/terms/identifier | "doi:10.1093/PROTEIN/GZT057"xsd:string |
| http://purl.uniprot.org/citations/24287187 | http://purl.org/dc/terms/identifier | "doi:10.1093/protein/gzt057"xsd:string |
| http://purl.uniprot.org/citations/24287187 | http://purl.uniprot.org/core/author | "Czjzek M."xsd:string |
| http://purl.uniprot.org/citations/24287187 | http://purl.uniprot.org/core/author | "Czjzek M."xsd:string |
| http://purl.uniprot.org/citations/24287187 | http://purl.uniprot.org/core/author | "Tran V."xsd:string |
| http://purl.uniprot.org/citations/24287187 | http://purl.uniprot.org/core/author | "Tran V."xsd:string |
| http://purl.uniprot.org/citations/24287187 | http://purl.uniprot.org/core/author | "Ropartz D."xsd:string |
| http://purl.uniprot.org/citations/24287187 | http://purl.uniprot.org/core/author | "Ropartz D."xsd:string |
| http://purl.uniprot.org/citations/24287187 | http://purl.uniprot.org/core/author | "Dion M."xsd:string |
| http://purl.uniprot.org/citations/24287187 | http://purl.uniprot.org/core/author | "Dion M."xsd:string |
| http://purl.uniprot.org/citations/24287187 | http://purl.uniprot.org/core/author | "Tellier C."xsd:string |
| http://purl.uniprot.org/citations/24287187 | http://purl.uniprot.org/core/author | "Tellier C."xsd:string |
| http://purl.uniprot.org/citations/24287187 | http://purl.uniprot.org/core/author | "Hendrickx J."xsd:string |
| http://purl.uniprot.org/citations/24287187 | http://purl.uniprot.org/core/author | "Hendrickx J."xsd:string |
| http://purl.uniprot.org/citations/24287187 | http://purl.uniprot.org/core/author | "Sanejouand Y.H."xsd:string |
| http://purl.uniprot.org/citations/24287187 | http://purl.uniprot.org/core/author | "Sanejouand Y.H."xsd:string |
| http://purl.uniprot.org/citations/24287187 | http://purl.uniprot.org/core/author | "Teze D."xsd:string |
| http://purl.uniprot.org/citations/24287187 | http://purl.uniprot.org/core/author | "Teze D."xsd:string |
| http://purl.uniprot.org/citations/24287187 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24287187 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24287187 | http://purl.uniprot.org/core/name | "Protein Eng. Des. Sel."xsd:string |
| http://purl.uniprot.org/citations/24287187 | http://purl.uniprot.org/core/name | "Protein Eng Des Sel"xsd:string |