| http://purl.uniprot.org/citations/24299952 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24299952 | http://www.w3.org/2000/01/rdf-schema#comment | "MicroRNAs have been proposed as novel regulators of vascular inflammation and dysfunction. This study aimed to evaluate the role of miR-149 in regulating the expression of key molecules associated with TNFα-induced endothelial activation. miR-149 was selected by in silico analysis and microRNA target prediction. Endothelial dysfunction was induced by TNFα treatment in Eahy926 endothelial cells and HUVEC. miR-149 level was evaluated by quantitative real time-polymerase chain reaction (RT-qPCR). Metalloproteinase-9 (MMP-9) was measured by zymography, Inducible Nitric Oxide Synthase (iNOS) by immunoblotting, Interleukin-6 (IL-6) and Interleukin-8 (IL-8) by ELISA. miR-149 regulatory effect was evaluated by gain-of-function technique upon miR-149 mimics transfection. TNFα down-modulated miR-149 level in Eahy926 and HUVEC. This effect was significantly abolished in Eahy926 by treatment with p38MAPK inhibitor. miR-149 mimic transfection counteracted the TNFα-induced expression of MMP-9, iNOS and IL-6. No effect was detected on IL-8 expression. Our results suggest that miR-149 represents an important new regulator of endothelial function through negative regulation of molecules associated with TNFα-induced endothelial dysfunction."xsd:string |
| http://purl.uniprot.org/citations/24299952 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbrc.2013.11.092"xsd:string |
| http://purl.uniprot.org/citations/24299952 | http://purl.uniprot.org/core/author | "Mura M."xsd:string |
| http://purl.uniprot.org/citations/24299952 | http://purl.uniprot.org/core/author | "Capponi S."xsd:string |
| http://purl.uniprot.org/citations/24299952 | http://purl.uniprot.org/core/author | "Mandich P."xsd:string |
| http://purl.uniprot.org/citations/24299952 | http://purl.uniprot.org/core/author | "Geroldi A."xsd:string |
| http://purl.uniprot.org/citations/24299952 | http://purl.uniprot.org/core/author | "Palmieri D."xsd:string |
| http://purl.uniprot.org/citations/24299952 | http://purl.uniprot.org/core/author | "Palombo D."xsd:string |
| http://purl.uniprot.org/citations/24299952 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24299952 | http://purl.uniprot.org/core/name | "Biochem Biophys Res Commun"xsd:string |
| http://purl.uniprot.org/citations/24299952 | http://purl.uniprot.org/core/pages | "246-251"xsd:string |
| http://purl.uniprot.org/citations/24299952 | http://purl.uniprot.org/core/title | "TNFalpha induces the expression of genes associated with endothelial dysfunction through p38MAPK-mediated down-regulation of miR-149."xsd:string |
| http://purl.uniprot.org/citations/24299952 | http://purl.uniprot.org/core/volume | "443"xsd:string |
| http://purl.uniprot.org/citations/24299952 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/24299952 |
| http://purl.uniprot.org/citations/24299952 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/24299952 |
| http://purl.uniprot.org/uniprot/P01375#attribution-B4F076A1BC4934507F2F0F0C2C97AD07 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/24299952 |