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http://purl.uniprot.org/citations/24309103http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/24309103http://www.w3.org/2000/01/rdf-schema#comment"

Introduction

Epithelial cell adhesion molecule (EpCAM) is expressed in tumors with an epithelial cell of origin, in a heterogeneous manner. Prostate cancer stem-like cells highly express EpCAM. However, little is known about how EpCAM is involved in the ability of cells to adapt to micro-environmental changes in available growth factors, which is one of the essential biological phenotypes of cancer stem-like cells (CSCs).

Methods

EpCAM-high and EpCAM-low subpopulations of cells were established from the prostate cancer cell line PC-3. Signal transductions in response to serum starvation, and on the exposure to EGF ligand or the specific inhibitor were analyzed in terms. Furthermore, we analyzed the expression level of amino acid transporters which contribute to the activation of mTOR signal between the two subgroups.

Results

EpCAM-high and EpCAM-low PC-3 subpopulations showed markedly different responses to serum starvation. EpCAM expression was positively correlated with activation of the mTOR and epithelial growth factor receptor (EGFR) signaling pathways. Furthermore, AMP-activated protein kinase (AMPK) was gradually de-activated in EpCAM-low PC-3 cells in the absence of serum.

Conclusions

EpCAM regulates the AMPK signaling pathway, essential for the response to growth factors characterized by EGF. LAT1, the amino acid transporter stabilized at the cellular membrane by EpCAM, is likely to be responsible for the difference in the susceptibility to EGF between EpCAM-high and EpCAM-low PC-3 cells."xsd:string
http://purl.uniprot.org/citations/24309103http://purl.org/dc/terms/identifier"doi:10.1016/j.bbrc.2013.11.093"xsd:string
http://purl.uniprot.org/citations/24309103http://purl.uniprot.org/core/author"Saya H."xsd:string
http://purl.uniprot.org/citations/24309103http://purl.uniprot.org/core/author"Yoshida G.J."xsd:string
http://purl.uniprot.org/citations/24309103http://purl.uniprot.org/core/date"2014"xsd:gYear
http://purl.uniprot.org/citations/24309103http://purl.uniprot.org/core/name"Biochem Biophys Res Commun"xsd:string
http://purl.uniprot.org/citations/24309103http://purl.uniprot.org/core/pages"239-245"xsd:string
http://purl.uniprot.org/citations/24309103http://purl.uniprot.org/core/title"EpCAM expression in the prostate cancer makes the difference in the response to growth factors."xsd:string
http://purl.uniprot.org/citations/24309103http://purl.uniprot.org/core/volume"443"xsd:string
http://purl.uniprot.org/citations/24309103http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/24309103
http://purl.uniprot.org/citations/24309103http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/24309103
http://purl.uniprot.org/uniprot/#_P16422-mappedCitation-24309103http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/24309103
http://purl.uniprot.org/uniprot/P16422http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/24309103