| http://purl.uniprot.org/citations/24324137 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24324137 | http://www.w3.org/2000/01/rdf-schema#comment | "During protein synthesis, tRNAs and their associated mRNA codons move sequentially on the ribosome from the A (aminoacyl) site to the P (peptidyl) site to the E (exit) site in a process catalyzed by a universally conserved ribosome-dependent GTPase [elongation factor G (EF-G) in prokaryotes and elongation factor 2 (EF-2) in eukaryotes]. Although the high-resolution structure of EF-G bound to the posttranslocation ribosome has been determined, the pretranslocation conformation of the ribosome bound with EF-G and A-site tRNA has evaded visualization owing to the transient nature of this state. Here we use electron cryomicroscopy to determine the structure of the 70S ribosome with EF-G, which is trapped in the pretranslocation state using antibiotic viomycin. Comparison with the posttranslocation ribosome shows that the small subunit of the pretranslocation ribosome is rotated by ∼12° relative to the large subunit. Domain IV of EF-G is positioned in the cleft between the body and head of the small subunit outwardly of the A site and contacts the A-site tRNA. Our findings suggest a model in which domain IV of EF-G promotes the translocation of tRNA from the A to the P site as the small ribosome subunit spontaneously rotates back from the hybrid, rotated state into the nonrotated posttranslocation state."xsd:string |
| http://purl.uniprot.org/citations/24324137 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.1311423110"xsd:string |
| http://purl.uniprot.org/citations/24324137 | http://purl.uniprot.org/core/author | "Brilot A.F."xsd:string |
| http://purl.uniprot.org/citations/24324137 | http://purl.uniprot.org/core/author | "Grigorieff N."xsd:string |
| http://purl.uniprot.org/citations/24324137 | http://purl.uniprot.org/core/author | "Ermolenko D.N."xsd:string |
| http://purl.uniprot.org/citations/24324137 | http://purl.uniprot.org/core/author | "Korostelev A.A."xsd:string |
| http://purl.uniprot.org/citations/24324137 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/24324137 | http://purl.uniprot.org/core/name | "Proc Natl Acad Sci U S A"xsd:string |
| http://purl.uniprot.org/citations/24324137 | http://purl.uniprot.org/core/pages | "20994-20999"xsd:string |
| http://purl.uniprot.org/citations/24324137 | http://purl.uniprot.org/core/title | "Structure of the ribosome with elongation factor G trapped in the pretranslocation state."xsd:string |
| http://purl.uniprot.org/citations/24324137 | http://purl.uniprot.org/core/volume | "110"xsd:string |
| http://purl.uniprot.org/citations/24324137 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/24324137 |
| http://purl.uniprot.org/citations/24324137 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/24324137 |
| http://purl.uniprot.org/uniprot/#_P0ADZ0-mappedCitation-24324137 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24324137 |
| http://purl.uniprot.org/uniprot/#_P0ADZ4-mappedCitation-24324137 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24324137 |
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| http://purl.uniprot.org/uniprot/#_P0AG63-mappedCitation-24324137 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24324137 |
| http://purl.uniprot.org/uniprot/#_P0C018-mappedCitation-24324137 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24324137 |
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| http://purl.uniprot.org/uniprot/#_P02359-mappedCitation-24324137 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24324137 |