| http://purl.uniprot.org/citations/24326668 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24326668 | http://www.w3.org/2000/01/rdf-schema#comment | "Heparin (HP), an important anticoagulant polysaccharide, is produced in a complex biosynthetic pathway in connective tissue-type mast cells. Both the structure and size of HP are critical factors determining the anticoagulation activity. A murine mastocytoma (MST) cell line was used as a model system to gain insight into this pathway. As reported, MST cells produce a highly sulfated HP-like polysaccharide that lacks anticoagulant activity (Montgomery RI, Lidholt K, Flay NW, Liang J, Vertel B, Lindahl U, Esko JD. 1992. Stable heparin-producing cell lines derived from the Furth murine mastocytoma. Proc Natl Acad Sci USA 89:11327-11331). Here, we show that transfection of MST cells with a retroviral vector containing heparan sulfate 3-O-sulfotransferase-1 (Hs3st1) restores anticoagulant activity. The MST lines express N-acetylglucosamine N-deacetylase/N-sulfotransferase-1, uronosyl 2-O-sulfotransferase and glucosaminyl 6-O-sulfotransferase-1, which are sufficient to make the highly sulfated HP. Overexpression of Hs3st1 in MST-10H cells resulted in a change in the composition of heparan sulfate (HS)/HP and CS/dermatan sulfate (DS) glycosaminoglycans. The cell-associated HS/HP closely resembles HP with 3-O-sulfo group-containing glucosamine residues and shows anticoagulant activity. This study contributes toward a better understanding of the HP biosynthetic pathway with the goal of providing tools to better control the biosynthesis of HP chains with different structures and activities."xsd:string |
| http://purl.uniprot.org/citations/24326668 | http://purl.org/dc/terms/identifier | "doi:10.1093/glycob/cwt108"xsd:string |
| http://purl.uniprot.org/citations/24326668 | http://purl.uniprot.org/core/author | "Li G."xsd:string |
| http://purl.uniprot.org/citations/24326668 | http://purl.uniprot.org/core/author | "Yang B."xsd:string |
| http://purl.uniprot.org/citations/24326668 | http://purl.uniprot.org/core/author | "Esko J.D."xsd:string |
| http://purl.uniprot.org/citations/24326668 | http://purl.uniprot.org/core/author | "Glass C.A."xsd:string |
| http://purl.uniprot.org/citations/24326668 | http://purl.uniprot.org/core/author | "Linhardt R.J."xsd:string |
| http://purl.uniprot.org/citations/24326668 | http://purl.uniprot.org/core/author | "Datta P."xsd:string |
| http://purl.uniprot.org/citations/24326668 | http://purl.uniprot.org/core/author | "Gemmill T.R."xsd:string |
| http://purl.uniprot.org/citations/24326668 | http://purl.uniprot.org/core/author | "Baik J.Y."xsd:string |
| http://purl.uniprot.org/citations/24326668 | http://purl.uniprot.org/core/author | "Sharfstein S.T."xsd:string |
| http://purl.uniprot.org/citations/24326668 | http://purl.uniprot.org/core/author | "Gasimli L."xsd:string |
| http://purl.uniprot.org/citations/24326668 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24326668 | http://purl.uniprot.org/core/name | "Glycobiology"xsd:string |
| http://purl.uniprot.org/citations/24326668 | http://purl.uniprot.org/core/pages | "272-280"xsd:string |
| http://purl.uniprot.org/citations/24326668 | http://purl.uniprot.org/core/title | "Bioengineering murine mastocytoma cells to produce anticoagulant heparin."xsd:string |
| http://purl.uniprot.org/citations/24326668 | http://purl.uniprot.org/core/volume | "24"xsd:string |
| http://purl.uniprot.org/citations/24326668 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/24326668 |
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| http://purl.uniprot.org/uniprot/O35310#attribution-BEE8D893E27820F167E410EC3CF57A01 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/24326668 |
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| http://purl.uniprot.org/uniprot/#_E0CYX6-mappedCitation-24326668 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24326668 |