Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/24333089http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/24333089http://www.w3.org/2000/01/rdf-schema#comment"

Background

The (C)ce(s) haplotype, mainly found in black individuals, contains two altered genes: a hybrid RHD-CE-D(s) gene segregated with a ce(s) allele of RHCE with two single nucleotide polymorphisms, c. 733C>G (p.Leu245Val) in exon 5 and c. 1006G>T (Gly336Cys) in exon 7. This haplotype could be responsible for false positive genotyping results in RhD-negative individuals and at a homozygous level lead to the loss of a high incidence antigen RH34. The aim of this study was to screen for the (C)ce(s) haplotype in Tunisian blood donors, given its clinico-biological importance.

Material and methods

Blood samples were randomly collected from blood donors in the blood transfusion centre of Sousse (Tunisia). A total of 356 RhD-positive and 44 RhD-negative samples were tested for the (C)ce(s) haplotype using two allele-specific primer polymerase chain reactions that detect c. 733C>G (p.Leu245Val) and c. 1006G>T (p. Gly336Cys) substitutions in exon 5 and 7 of the RHCE gene. In addition, the presence of the D-CE hybrid exon 3 was evaluated using a sequence-specific primer polymerase chain reaction.

Results

Among the 400 individuals only five exhibited the (C)ce(s) haplotype in heterozygosity, for a frequency of 0.625%. On the basis of the allele-specific primer polymerase chain reaction results, the difference in (C)ce(s) haplotype frequency was not statistically significant between RhD-positive and RhD-negative blood donors.

Discussion

These data showed the presence of the (C)ce(s) haplotype at a low frequency (0.625%) compared to that among Africans in whom it is common. Nevertheless, the presence of RHD-CE-D(s) in Tunisians, even at a lower frequency, should be considered in the development of a molecular genotyping strategy for Rh genes, to ensure better management of the prevention of alloimmunisation."xsd:string
http://purl.uniprot.org/citations/24333089http://purl.org/dc/terms/identifier"doi:10.2450/2013.0153-13"xsd:string
http://purl.uniprot.org/citations/24333089http://purl.uniprot.org/core/author"Abdelkefi S."xsd:string
http://purl.uniprot.org/citations/24333089http://purl.uniprot.org/core/author"Chakroun T."xsd:string
http://purl.uniprot.org/citations/24333089http://purl.uniprot.org/core/author"Houissa B."xsd:string
http://purl.uniprot.org/citations/24333089http://purl.uniprot.org/core/author"Romdhane H."xsd:string
http://purl.uniprot.org/citations/24333089http://purl.uniprot.org/core/author"Moussa H."xsd:string
http://purl.uniprot.org/citations/24333089http://purl.uniprot.org/core/author"Ghommen N."xsd:string
http://purl.uniprot.org/citations/24333089http://purl.uniprot.org/core/author"Jemni S.Y."xsd:string
http://purl.uniprot.org/citations/24333089http://purl.uniprot.org/core/date"2014"xsd:gYear
http://purl.uniprot.org/citations/24333089http://purl.uniprot.org/core/name"Blood Transfus"xsd:string
http://purl.uniprot.org/citations/24333089http://purl.uniprot.org/core/pages"405-409"xsd:string
http://purl.uniprot.org/citations/24333089http://purl.uniprot.org/core/title"(C)ce(s) haplotype screening in Tunisian blood donors."xsd:string
http://purl.uniprot.org/citations/24333089http://purl.uniprot.org/core/volume"12"xsd:string
http://purl.uniprot.org/citations/24333089http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/24333089
http://purl.uniprot.org/citations/24333089http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/24333089
http://purl.uniprot.org/uniprot/#_A0A089VLV6-mappedCitation-24333089http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/24333089
http://purl.uniprot.org/uniprot/#_D0AB03-mappedCitation-24333089http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/24333089
http://purl.uniprot.org/uniprot/#_D0AB04-mappedCitation-24333089http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/24333089
http://purl.uniprot.org/uniprot/#_A0A078BQE0-mappedCitation-24333089http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/24333089
http://purl.uniprot.org/uniprot/#_A0A078N0E5-mappedCitation-24333089http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/24333089
http://purl.uniprot.org/uniprot/#_A0A0K0QTI0-mappedCitation-24333089http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/24333089
http://purl.uniprot.org/uniprot/#_A0A0D3QSK8-mappedCitation-24333089http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/24333089
http://purl.uniprot.org/uniprot/#_A0A0D4CYF7-mappedCitation-24333089http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/24333089
▶