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http://purl.uniprot.org/citations/24342833http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/24342833http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/24342833http://www.w3.org/2000/01/rdf-schema#comment"

Background

We have previously reported that β-catenin is post-translationally modified with a single O-linked attachment of β-N-acetyl-glucosamine (O-GlcNAc). We showed that O-GlcNAc regulated β-catenin's subcellular localization and transcriptional activity.

Objective

The objectives of this investigation were to identify the putative O-GlcNAc sites of β-catenin and the relevance of identified sites in the regulation of β-catenin's localization and transcriptional activity.

Method

Missense mutations were introduced to potential O-GlcNAc sites of pEGFP-C2-N-Terminal- or pEGFP-C2-Wild Type-β-catenin by site-directed mutagenesis. We determined the levels of O-GlcNAc-β-catenin, subcellular localization, interaction with binding partners and transcriptional activity of the various constructs.

Results

Serine 23 of β-catenin was determined as a site for O-GlcNAc modification which regulated its subcellular distribution, its interactions with cellular partners and consequently its transcriptional activity.

Significance

O-GlcNAcylation of Serine 23 is a novel regulatory modification for β-catenin's subcellular localization and transcriptional activity. This study is the first report to characterize site specific regulation of β-catenin by the O-GlcNAc modification."xsd:string
http://purl.uniprot.org/citations/24342833http://purl.org/dc/terms/identifier"doi:10.1016/j.yexcr.2013.11.021"xsd:string
http://purl.uniprot.org/citations/24342833http://purl.org/dc/terms/identifier"doi:10.1016/j.yexcr.2013.11.021"xsd:string
http://purl.uniprot.org/citations/24342833http://purl.uniprot.org/core/author"Garcia E."xsd:string
http://purl.uniprot.org/citations/24342833http://purl.uniprot.org/core/author"Garcia E."xsd:string
http://purl.uniprot.org/citations/24342833http://purl.uniprot.org/core/author"Hao L."xsd:string
http://purl.uniprot.org/citations/24342833http://purl.uniprot.org/core/author"Hao L."xsd:string
http://purl.uniprot.org/citations/24342833http://purl.uniprot.org/core/author"Huang Y.H."xsd:string
http://purl.uniprot.org/citations/24342833http://purl.uniprot.org/core/author"Huang Y.H."xsd:string
http://purl.uniprot.org/citations/24342833http://purl.uniprot.org/core/author"Ha J.R."xsd:string
http://purl.uniprot.org/citations/24342833http://purl.uniprot.org/core/author"Ha J.R."xsd:string
http://purl.uniprot.org/citations/24342833http://purl.uniprot.org/core/author"Persad S."xsd:string
http://purl.uniprot.org/citations/24342833http://purl.uniprot.org/core/author"Persad S."xsd:string
http://purl.uniprot.org/citations/24342833http://purl.uniprot.org/core/author"Venkateswaran G."xsd:string
http://purl.uniprot.org/citations/24342833http://purl.uniprot.org/core/author"Venkateswaran G."xsd:string
http://purl.uniprot.org/citations/24342833http://purl.uniprot.org/core/date"2014"xsd:gYear
http://purl.uniprot.org/citations/24342833http://purl.uniprot.org/core/date"2014"xsd:gYear
http://purl.uniprot.org/citations/24342833http://purl.uniprot.org/core/name"Exp. Cell Res."xsd:string
http://purl.uniprot.org/citations/24342833http://purl.uniprot.org/core/name"Exp. Cell Res."xsd:string
http://purl.uniprot.org/citations/24342833http://purl.uniprot.org/core/pages"153-166"xsd:string
http://purl.uniprot.org/citations/24342833http://purl.uniprot.org/core/pages"153-166"xsd:string
http://purl.uniprot.org/citations/24342833http://purl.uniprot.org/core/title"beta-catenin is O-GlcNAc glycosylated at Serine 23: implications for beta-catenin's subcellular localization and transactivator function."xsd:string
http://purl.uniprot.org/citations/24342833http://purl.uniprot.org/core/title"beta-catenin is O-GlcNAc glycosylated at Serine 23: implications for beta-catenin's subcellular localization and transactivator function."xsd:string
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