| http://purl.uniprot.org/citations/24343376 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24343376 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24343376 | http://www.w3.org/2000/01/rdf-schema#comment | "In eukaryotes, the 26S proteasome degrades ubiquitinylated proteins in an ATP-dependent manner. Archaea mediate a form of post-translational modification of proteins termed sampylation that resembles ubiquitinylation. Sampylation was identified in Haloferax volcanii, a moderate halophilic archaeon that synthesizes homologs of 26S proteasome subunits including 20S core particles and regulatory particle triple-A ATPases (Rpt)-like proteasome-associated nucleotidases (PAN-A/1 and PAN-B/2). To determine whether sampylated proteins associate with the Rpt subunit homologs, PAN-A/1 was purified to homogeneity from Hfx. volcanii and analyzed for its subunit stoichiometry, nucleotide-hydrolyzing activity and binding to sampylated protein targets. PAN-A/1 was found to be associated as a dodecamer (630 kDa) with a configuration in TEM suggesting a complex of two stacked hexameric rings. PAN-A/1 had high affinity for ATP (K m of ~0.44 mM) and hydrolyzed this nucleotide with a specific activity of 0.33 ± 0.1 μmol Pi/h per mg protein and maximum at 42 °C. PAN-A1 was stabilized by 2 M salt with a decrease in activity at lower concentrations of salt that correlated with dissociation of the dodecamer into trimers to monomers. Binding of PAN-A/1 to a sampylated protein was demonstrated by modification of a far Western blotting technique (derived from the standard Western blot method to detect protein-protein interaction in vitro) for halophilic proteins. Overall, our results support a model in which sampylated proteins associate with the PAN-A/1 AAA+ ATPase in proteasome-mediated proteolysis and/or protein remodeling and provide a method for assay of halophilic protein-protein interactions."xsd:string |
| http://purl.uniprot.org/citations/24343376 | http://purl.org/dc/terms/identifier | "doi:10.1007/s00792-013-0615-8"xsd:string |
| http://purl.uniprot.org/citations/24343376 | http://purl.org/dc/terms/identifier | "doi:10.1007/s00792-013-0615-8"xsd:string |
| http://purl.uniprot.org/citations/24343376 | http://purl.uniprot.org/core/author | "Maupin-Furlow J.A."xsd:string |
| http://purl.uniprot.org/citations/24343376 | http://purl.uniprot.org/core/author | "Maupin-Furlow J.A."xsd:string |
| http://purl.uniprot.org/citations/24343376 | http://purl.uniprot.org/core/author | "Wu Y."xsd:string |
| http://purl.uniprot.org/citations/24343376 | http://purl.uniprot.org/core/author | "Wu Y."xsd:string |
| http://purl.uniprot.org/citations/24343376 | http://purl.uniprot.org/core/author | "Prunetti L."xsd:string |
| http://purl.uniprot.org/citations/24343376 | http://purl.uniprot.org/core/author | "Prunetti L."xsd:string |
| http://purl.uniprot.org/citations/24343376 | http://purl.uniprot.org/core/author | "Miranda H.V."xsd:string |
| http://purl.uniprot.org/citations/24343376 | http://purl.uniprot.org/core/author | "Miranda H.V."xsd:string |
| http://purl.uniprot.org/citations/24343376 | http://purl.uniprot.org/core/author | "Hepowit N.L."xsd:string |
| http://purl.uniprot.org/citations/24343376 | http://purl.uniprot.org/core/author | "Hepowit N.L."xsd:string |
| http://purl.uniprot.org/citations/24343376 | http://purl.uniprot.org/core/author | "Barrueto L."xsd:string |
| http://purl.uniprot.org/citations/24343376 | http://purl.uniprot.org/core/author | "Barrueto L."xsd:string |
| http://purl.uniprot.org/citations/24343376 | http://purl.uniprot.org/core/author | "Kelly K."xsd:string |
| http://purl.uniprot.org/citations/24343376 | http://purl.uniprot.org/core/author | "Kelly K."xsd:string |
| http://purl.uniprot.org/citations/24343376 | http://purl.uniprot.org/core/author | "Reuter C.J."xsd:string |
| http://purl.uniprot.org/citations/24343376 | http://purl.uniprot.org/core/author | "Reuter C.J."xsd:string |
| http://purl.uniprot.org/citations/24343376 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24343376 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24343376 | http://purl.uniprot.org/core/name | "Extremophiles"xsd:string |
| http://purl.uniprot.org/citations/24343376 | http://purl.uniprot.org/core/name | "Extremophiles"xsd:string |