http://purl.uniprot.org/citations/24356419 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/24356419 | http://www.w3.org/2000/01/rdf-schema#comment | "BackgroundAngiogenin (ANG) can translocate to the target cell nucleus and accumulate in the nucleolus to enhance rRNA transcription, thus promoting cell proliferation. However, the regulation of ANG-enhanced rRNA transcription remains unknown. Previously we identified phospholipid scramblase 1 (PLSCR1) as a potential ANG-interacting protein in yeast two-hybrid screening.MethodsThe interaction was re-confirmed in yeast cells and further verified by in vitro pull down, in vivo co-immunoprecipitation (Co-IP), fluorescent resonance energy transfer (FRET) and immunofluorescence analyses. The rRNA transcription level was determined by real-time quantitative PCR and Northern blot.ResultsPLSCR1 was identified as a novel ANG-interacting protein. Notably, PLSCR1 interacted with ANG in the cell nucleus and regulated rRNA transcription. Furthermore, depletion of cellular ANG expression abolished PLSCR1-enhanced rRNA transcription, which could be rescued by exogenous ANG.ConclusionOur data suggest that PLSCR1 positively regulates rRNA transcription through interacting with ANG, thus deepening our understanding on rRNA transcription regulation."xsd:string |
http://purl.uniprot.org/citations/24356419 | http://purl.org/dc/terms/identifier | "doi:10.1159/000356604"xsd:string |
http://purl.uniprot.org/citations/24356419 | http://purl.uniprot.org/core/author | "Dong H."xsd:string |
http://purl.uniprot.org/citations/24356419 | http://purl.uniprot.org/core/author | "Kang L."xsd:string |
http://purl.uniprot.org/citations/24356419 | http://purl.uniprot.org/core/author | "Xu Z."xsd:string |
http://purl.uniprot.org/citations/24356419 | http://purl.uniprot.org/core/author | "Zhu J."xsd:string |
http://purl.uniprot.org/citations/24356419 | http://purl.uniprot.org/core/author | "Sheng J."xsd:string |
http://purl.uniprot.org/citations/24356419 | http://purl.uniprot.org/core/author | "Ang J."xsd:string |
http://purl.uniprot.org/citations/24356419 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
http://purl.uniprot.org/citations/24356419 | http://purl.uniprot.org/core/name | "Cell Physiol Biochem"xsd:string |
http://purl.uniprot.org/citations/24356419 | http://purl.uniprot.org/core/pages | "1695-1706"xsd:string |
http://purl.uniprot.org/citations/24356419 | http://purl.uniprot.org/core/title | "Phospholipid scramblase 1 functionally interacts with angiogenin and regulates angiogenin-enhanced rRNA transcription."xsd:string |
http://purl.uniprot.org/citations/24356419 | http://purl.uniprot.org/core/volume | "32"xsd:string |
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