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| http://purl.uniprot.org/citations/2436975 | http://www.w3.org/2000/01/rdf-schema#comment | "We have constructed a new series of the pUC-derived plasmids with an extended polylinker obtained from M13tg131 [Kieny et al., Gene 26 (1983) 91-99]. These vectors allowed us to design a simplified version of the method of Okayama and Berg [Mol. Cell. Biol. 2 (1982) 161-170] for establishing complete cDNA libraries. Improvements included easy recovery of the inserted cDNA due to the extended polylinkers; use of these vectors for gene expression in Escherichia coli, and amenability to supercoil sequencing with the universal primers of the M13 system [Chen and Seeburg, DNA 4 (1985) 165-170], which speeds up the identification of positive clones. Moreover, there is no need for an additional linker fragment, as was required by the Okayama and Berg [Mol. Cell. Biol. 2 (1982) 161-170] method. We have successfully used this system to obtain cDNA clones coding for the different chains of the large basement membrane proteins type IV collagen and laminin."xsd:string |
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| http://purl.uniprot.org/citations/2436975 | http://purl.uniprot.org/core/author | "Oberbaumer I."xsd:string |
| http://purl.uniprot.org/citations/2436975 | http://purl.uniprot.org/core/date | "1986"xsd:gYear |
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| http://purl.uniprot.org/citations/2436975 | http://purl.uniprot.org/core/title | "New pUC-derived expression vectors for rapid construction of cDNA libraries."xsd:string |
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