| http://purl.uniprot.org/citations/24389104 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24389104 | http://www.w3.org/2000/01/rdf-schema#comment | "We have developed a technique, called Ubiquitin Ligase Substrate Trapping, for the isolation of ubiquitinated substrates in complex with their ubiquitin ligase (E3). By fusing a ubiquitin-associated (UBA) domain to an E3 ligase, we were able to selectively purify the polyubiquitinated forms of E3 substrates. Using ligase traps of eight different F box proteins (SCF specificity factors) coupled with mass spectrometry, we identified known, as well as previously unreported, substrates. Polyubiquitinated forms of candidate substrates associated with their cognate F box partner, but not other ligase traps. Interestingly, the four most abundant candidate substrates identified for the F box protein Saf1 were all vacuolar/lysosomal proteins. Analysis of one of these substrates, Prb1, showed that Saf1 selectively promotes ubiquitination of the unprocessed form of the zymogen. This suggests that Saf1 is part of a pathway that targets protein precursors for proteasomal degradation."xsd:string |
| http://purl.uniprot.org/citations/24389104 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.molcel.2013.12.003"xsd:string |
| http://purl.uniprot.org/citations/24389104 | http://purl.uniprot.org/core/author | "Maiolica A."xsd:string |
| http://purl.uniprot.org/citations/24389104 | http://purl.uniprot.org/core/author | "Seller C.A."xsd:string |
| http://purl.uniprot.org/citations/24389104 | http://purl.uniprot.org/core/author | "Toczyski D.P."xsd:string |
| http://purl.uniprot.org/citations/24389104 | http://purl.uniprot.org/core/author | "Mark K.G."xsd:string |
| http://purl.uniprot.org/citations/24389104 | http://purl.uniprot.org/core/author | "Simonetta M."xsd:string |
| http://purl.uniprot.org/citations/24389104 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24389104 | http://purl.uniprot.org/core/name | "Mol Cell"xsd:string |
| http://purl.uniprot.org/citations/24389104 | http://purl.uniprot.org/core/pages | "148-161"xsd:string |
| http://purl.uniprot.org/citations/24389104 | http://purl.uniprot.org/core/title | "Ubiquitin ligase trapping identifies an SCF(Saf1) pathway targeting unprocessed vacuolar/lysosomal proteins."xsd:string |
| http://purl.uniprot.org/citations/24389104 | http://purl.uniprot.org/core/volume | "53"xsd:string |
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